Characterization of eight barley xantha-f mutants deficient in magnesium chelatase

Plant Physiology and Biochemistry : PPB
Ulf OlssonMats Hansson

Abstract

Magnesium chelatase (EC 6.6.1.1) catalyses the insertion of magnesium into protoporphyrin IX, the first unique step of the chlorophyll biosynthetic pathway. The enzyme is composed of three different subunits of approximately 40, 70 and 140 kDa. In barley (Hordeum vulgare L.) the subunits are encoded by the genes Xantha-h, Xantha-g and Xantha-f. In the 1950s, eight induced xantha-f mutants were isolated. In this work we characterized these mutations at the DNA level and provided explanations for their phenotypes. The xantha-f10 mutation is a 3 bp deletion, resulting in a polypeptide lacking the glutamate residue at position 424. The leaky mutation xantha-f26 has a missense mutation leading to a M632R exchange. The xantha-f27 and -f40 are deletions of 14 and 2 bp, respectively, resulting in truncated polypeptides of 1104 and 899 amino acid residues, respectively. Mutation xantha-f41 is an in-frame deletion that removes A439, L440, Q441 and V442 from the resulting protein. Mutation xantha-f58 is most likely a deletion of the whole Xantha-f gene, as no DNA fragments could be detected by PCR or southern blot experiments. The slightly leaky xantha-f60 and non-leaky -f68 mutations each have a missense mutation causing a P393L and G794...Continue Reading

References

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Citations

Apr 28, 2012·Plant & Cell Physiology·Andre Hubertus MuellerMats Hansson
Jan 30, 2009·Plant Physiology·André H Müller, Mats Hansson
May 22, 2004·European Journal of Biochemistry·Vanessa LakeMats Hansson
Mar 13, 2014·Plant Physiology and Biochemistry : PPB·Ilka BraumannMats Hansson
Apr 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kamila RzeznickaMats Hansson
Jul 4, 2007·Plant Physiology and Biochemistry : PPB·Shakhira ZakhrabekovaMats Hansson
Aug 28, 2004·Plant Physiology and Biochemistry : PPB·Rena GadjievaMats Hansson
Nov 29, 2005·Plant Physiology and Biochemistry : PPB·Rena GadjievaMats Hansson
Jul 27, 2005·Biochemical Society Transactions·M Moulin, A G Smith

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