Characterization of electrostatic and nonelectrostatic components of protein--membrane binding interactions

Biochemistry
J B HeymannW A Cramer

Abstract

A general method was developed to determine the thermodynamic parameters for the interaction of protein with membranes. Protein intrinsic tryptophan fluorescence was quenched by titration with large unilamellar vesicles containing 9,10-dibrominated distearoylphosphatidylcholine (Br4-DSPC) or a small amount of trinitrophenylphosphatidylethanolamine (TNP-PE), Binding was modeled as a bimolecular reaction of free protein with a unit of "n" lipid molecules and a dissociation constant, Kd. The contribution of residual fluorescence and light scattering could be eliminated by using the second derivative of the titration function as the basis for calculations. For the binding of C-terminal channel domain polypeptides(178-190 residues) of the colicin El ion channel, n=50-60 and Kd=2-3 nM at pH 4, ionic strength, I=0.12 M, and anionic lipid content = 40% (surface potential, psi o =-30 mV), conditions for which the protein has high activity. Values of n = 95 and 210 for the binding of a C-terminal 293-residue colicin fragment and the 522 residue intact colicin E1 molecule scale qualitatively according to the increase in molecular size. General methods are presented to distinguish the electrostatic (delta G el) and nonelectrostatic (delta ...Continue Reading

References

Apr 5, 1992·Journal of Molecular Biology·M W ParkerD Tsernoglou
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·S McLaughlin
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·W A CramerC V Stauffacher

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Citations

Apr 1, 1998·Protein Science : a Publication of the Protein Society·Y KimW A Cramer
Feb 25, 2003·Chemistry and Physics of Lipids·Daniel AllendeThomas J McIntosh
May 16, 2000·Chemistry and Physics of Lipids·M Langner, K Kubica
Sep 8, 1998·Current Opinion in Structural Biology·R M StroudD Freymann
Feb 7, 2006·Biochemistry. Biokhimii︠a︡·A A SobkoY N Antonenko
Jan 12, 2007·Biological Chemistry·Kerstin ElfrinkDetlev Riesner
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S D ZakharovW A Cramer
Sep 1, 2012·Annual Review of Genetics·Karen S Jakes, William A Cramer
Jun 6, 2014·Chemical Reviews·Francois-Xavier TheilletPhilipp Selenko
Nov 3, 2004·Biochimica Et Biophysica Acta·Stanislav D ZakharovWilliam A Cramer
May 3, 2006·Journal of Molecular Biology·Guruvasuthevan R ThuduppathyR Blake Hill
Aug 9, 2008·Journal of Molecular Biology·Jorge Alegre-CebolladaAlvaro Martínez-del-Pozo
Nov 1, 2002·Biochimica Et Biophysica Acta·Stanislav D Zakharov, William A Cramer
Jan 7, 2000·Journal of Molecular Biology·M LindebergW A Cramer
Sep 30, 2017·Biotechnology and Applied Biochemistry·Almerinda Di VenereGiampiero Mei
Aug 13, 2004·Proceedings. Biological Sciences·H G E HentschelStuart A Newman
Apr 26, 2003·The Journal of Biological Chemistry·Abdiwahab A Musse, A Rod Merrill
Feb 13, 2021·International Journal of Molecular Sciences·Francesca RipantiGiampiero Mei

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