Characterization of endophytic Bacillus strains from tomato plants (Lycopersicon esculentum) displaying antifungal activity against Botrytis cinerea Pers

World Journal of Microbiology & Biotechnology
Asma KefiF Limam

Abstract

Eighty endophytic bacteria were isolated from healthy tissues of roots, stems, leaves and fruits of tomato plants (Lycopersicon esculentum). Four strains, named BL1, BT5, BR8 and BF11 were selected for their antagonism against Botrytis cinerea, a phytopathogenic fungus responsible of gray mold in several important crops, with growth inhibitory activity ranging from 27 to 53%. Morphological, biochemical, and molecular parameters as 16S rDNA sequencing demonstrated that the selected bacterial strains were related to Bacillus species which are known to produce and secrete a lot of lipopeptides with strong inhibitory effect against pathogen mycelial growth. Electrospray mass spectrometry analysis showed that these strains produced heterogeneous mixture of antibiotics belonging to fengycin and surfactin for BL1 and BT5, to iturin and surfactin for BR8, to bacillomycin D, fengycin and surfactin for BF11. Furthermore, these bacteria exhibited biocontrol potential by reducing the disease severity when tested on detached leaflets. Based on their antifungal activity against Botrytis cinerea, these strains could be used for biological control of plant diseases.

References

Jan 1, 1966·Antimicrobial Agents and Chemotherapy·E A KaczkaF J Wolf
Feb 13, 1999·FEMS Microbiology Letters·E A Emmert, J Handelsman
Apr 20, 2002·Applied Biochemistry and Biotechnology·E AkpaP Thonart
Sep 6, 2003·Applied and Environmental Microbiology·Justin T Coombs, Christopher M M Franco
Oct 14, 2004·Journal of Applied Microbiology·P I KimY-T Chi
Jan 29, 2005·Journal of Basic Microbiology·M RajkumarKui Jae Lee
Nov 1, 1974·Antimicrobial Agents and Chemotherapy·D Raahave
Nov 1, 1996·Applied and Environmental Microbiology·O Asaka, M Shoda
Mar 29, 2011·International Journal of Systematic and Evolutionary Microbiology·Yun Zeng ZhangWen Xin Chen
Aug 31, 2013·World Journal of Microbiology & Biotechnology·L Kalai-GramiF Limam

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Citations

Aug 5, 2016·Rapid Communications in Mass Spectrometry : RCM·Gabriella FavaroBarbara Baldan
Sep 19, 2016·Applied Biochemistry and Biotechnology·Mohamed Amine AounallahOlfa Tabbene
Oct 30, 2018·Frontiers in Plant Science·Sofia D Carvalho, José A Castillo
Jul 28, 2020·Archives of Microbiology·Silvina M Y LópezPedro A Balatti
Sep 27, 2018·Applied Biochemistry and Biotechnology·Siwar SoussiFerid Limam
Jul 30, 2021·Frontiers in Microbiology·Ashraf Al AshhabGidon Winters

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