Characterization of Endothelial and Smooth Muscle Cells From Different Canine Vessels

Frontiers in Physiology
Loes A OosterhoffFrank G van Steenbeek

Abstract

Vasculature performs a critical function in tissue homeostasis, supply of oxygen and nutrients, and the removal of metabolic waste products. Vascular problems are implicated in a large variety of pathologies and accurate in vitro models resembling native vasculature are of great importance. Unfortunately, existing in vitro models do not sufficiently reflect their in vivo counterpart. The complexity of vasculature requires the examination of multiple cell types including endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), as well as vessel location in the body from which they originate. The use of canine blood vessels provides a way to study vasculature with similar vessel size and physiology compared to human vasculature. We report an isolation procedure that provides the possibility to isolate both the endothelial and smooth muscle cells from the same vessels simultaneously, enabling new opportunities in investigating vasculature behavior. Canine primary ECs and VSMCs were isolated from the vena cava, vena porta and aorta. All tissue sources were derived from three donors for accurate comparison and to reduce inter-animal variation. The isolation and purification of the two distinct cell types was confirmed by mo...Continue Reading

References

Sep 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C L IvesL V McIntire
Aug 1, 1985·Journal of the American College of Cardiology·A C Gittenberger-de GrootD F Patterson
May 26, 1998·Biochemical and Biophysical Research Communications·E SegiA Ichikawa
Feb 7, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T KorffH G Augustin
Feb 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·C D LoftinR Langenbach
Jun 5, 2003·Nature Medicine·Rakesh K Jain
Aug 2, 2003·Journal of Cellular Physiology·Carine Michiels
May 26, 2009·Nucleic Acids Research·Jing ChenAnil G Jegga
Apr 25, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Bo ZhangShunan Ye
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
May 29, 2013·Journal of Tissue Engineering and Regenerative Medicine·Sandra StrassburgNestor Torio-Padron
May 17, 2014·Assay and Drug Development Technologies·Rasheena EdmondsonLiju Yang
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Apr 11, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maximilian HeissThomas Korff
Apr 12, 2016·The Veterinary Journal·L Van den Bossche, F G van Steenbeek
Jun 15, 2016·BMC Veterinary Research·Marjolein L den ToomHenri C M Heuven
Jun 22, 2016·Animal Genetics·F G van SteenbeekH Lohi
Oct 4, 2016·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·P Laborda-VidalT W Maddox
Oct 12, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yi YangZiming Dong
Oct 30, 2016·Trends in Biotechnology·Sandra HauserJens Pietzsch
Dec 3, 2016·Journal of Visualized Experiments : JoVE·Loes A OosterhoffFrank G van Steenbeek
Dec 8, 2016·Tissue Engineering. Part C, Methods·Minu Karthika GanesanPeter Petzelbauer
Jan 8, 2017·Annals of the Rheumatic Diseases·Mirko ManettiMarco Matucci-Cerinic
Sep 28, 2017·Bioengineering·Seunggyu KimJessie S Jeon
Oct 8, 2017·Scientific Reports·Agata RadwanskaEllen Van Obberghen-Schilling
Dec 8, 2017·Physiological Genomics·Jonathan W NelsonAnthony P Barnes
Jan 10, 2018·Advances in Pharmacology·Jaroslava Halper
Jul 8, 2018·Nature Communications·Lucia NatarelliAndreas Schober

❮ Previous
Next ❯

Citations

May 25, 2021·Frontiers in Pharmacology·Pragyan AcharyaRalf Weiskirchen
Aug 28, 2021·International Journal of Molecular Sciences·Kinga WilkusClaudine Kieda

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE118029
GSE114805
GSE104530
GSE76743

Methods Mentioned

BETA
RNA-Seq
Assay
FACS

Software Mentioned

Sambamba
STAR
DESeq2
ToppFun
HTSeq
edgeR
AddOrReplaceReadGroups
count
ImageJ

Related Concepts

Related Feeds

Bioreactors (ASM)

Bioreactors are important devices or systems that utilize living cells or enzymes for a chemical process. These devices carefully control and monitor the environment factors such as pH, nutrients level, and temperature, which can affect the yield in a given process. Discover the latest research here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Jen-Tsan ChiPatrick O Brown
Arteriosclerosis, Thrombosis, and Vascular Biology
Achim LotherLutz Hein
Biotechnology Journal
Maureen WanjareSharon Gerecht
© 2022 Meta ULC. All rights reserved