Characterization of enriched CD34+ cells from healthy volunteers and those from patients treated with chemotherapy plus granulocyte colony-stimulating factor (G-CSF)

Stem Cells
T SuzukiY Miura

Abstract

CD34+ cells were enriched, using a panning method, from peripheral blood (PB) and bone marrow (BM) of healthy volunteers and of patients treated with chemotherapy plus granulocyte colony-stimulating factor (G-CSF). In healthy volunteers, PB CD34+ cells expressed CD33 and CD13 at a higher frequency than BM CD34+ cells, and PB CD34+ cells contained a greater number of burst-forming units-erythroid (BFU-E) than colony-forming units granulocyte-macrophage (CFU-GM). Administration of G-CSF to healthy volunteers induced a marked increase in the number of PB CD34+ cells, although the proportions of those expressing CD33, CD13, and c-kit among these cells as well as colony-forming ability were not changed before and after G-CSF administration. There were no significant differences in surface antigens on PB CD34+ cells between healthy volunteers and patients after chemotherapy plus G-CSF, except for low expression of c-kit in the PB of patients. However, PB CD34+ cells from patients contained almost the same number of CFU-GM as BFU-E. These results indicate that there were clear differences in the features of CD34+ cells from BM and from PB, and between healthy volunteers and patients after chemotherapy plus G-CSF. Enriched CD34+ cells ...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A G LearyM Ogawa
Jun 1, 1989·British Journal of Haematology·J ReiffersJ M Boiron

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Citations

Jan 1, 1997·Stem Cells·M EderA Ganser
Dec 1, 1995·Stem Cells·A Gee
Dec 1, 1995·Stem Cells·W I BensingerC D Buckner
Sep 13, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Ayhan DonmezNihal Gokmen
Feb 13, 2001·British Journal of Haematology·F de BoerG J Schuurhuis
Jan 1, 1996·Stem Cells·T DemirerW I Bensinger
Aug 5, 2006·Stem Cells·Shigeru Chiba

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