PMID: 9176674May 1, 1997Paper

Characterization of epithelial primary cultures from human conjunctiva

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Yolanda DieboldJ C Pastor

Abstract

Primary cultures of human epithelial cells from normal conjunctiva were developed and characterized to determine whether they retained epithelial characteristics. Conjunctival explants were obtained from the upper fornix of healthy donors and cultured in supplemented DMEM/F-12 medium for 5 days. The epithelial outgrowth was maintained for an additional 10 days. Primary cultures were then processed for light microscopy, transmission and scanning electron microscopy (TEM, SEM), and immunocytochemistry. They exhibited typical features of conjunctival epithelium on light microscopy (polygonal morphology, intimate cohesion, production of mucins), TEM (abundant desmosomes, keratin bundles, granules, microvilli), SEM (polygonal shape, microvilli, intimate cohesion), and immunocytochemistry (positivity for the receptor of epidermal growth factor, desmosomal proteins, and cytokeratins). In conclusion, primary cultures developed from normal human conjunctiva maintained the epithelial characteristics in vitro. Because the conjunctiva is a major component of the anterior ocular surface, we propose this in vitro system as suitable for physiopathologic and toxicologic studies.

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Citations

Jan 25, 2008·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·K KasperG Geerling
Jan 20, 2004·Experimental Eye Research·Darlene A Dartt
Nov 9, 2004·Survey of Ophthalmology·Suphi TaneriDimitri T Azar
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Dec 19, 2020·Pharmaceutics·Laura García-Posadas, Yolanda Diebold
Aug 29, 2021·Pharmaceutics·Yolanda Diebold, Laura García-Posadas

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