Characterization of Erwinia amylovora strains from different host plants using repetitive-sequences PCR analysis, and restriction fragment length polymorphism and short-sequence DNA repeats of plasmid pEA29

Journal of Applied Microbiology
D BarionoviM Scortichini

Abstract

The three main aims of the study were the assessment of the genetic relationship between a deviating Erwinia amylovora strain isolated from Amelanchier sp. (Maloideae) grown in Canada and other strains from Maloideae and Rosoideae, the investigation of the variability of the PstI fragment of the pEA29 plasmid using restriction fragment length polymorphism (RFLP) analysis and the determination of the number of short-sequence DNA repeats (SSR) by DNA sequence analysis in representative strains. Ninety-three strains obtained from 12 plant genera and different geographical locations were examined by repetitive-sequences PCR using Enterobacterial Repetitive Intergenic Consensus, BOX and Repetitive Extragenic Palindromic primer sets. Upon the unweighted pair group method with arithmetic mean analysis, a deviating strain from Amelanchier sp. was analysed using amplified ribosomal DNA restriction analysis (ARDRA) analysis and the sequencing of the 16S rDNA gene. This strain showed 99% similarity to other E. amylovora strains in the 16S gene and the same banding pattern with ARDRA. The RFLP analysis of pEA29 plasmid using MspI and Sau3A restriction enzymes showed a higher variability than that previously observed and no clear-cut groupi...Continue Reading

References

Nov 1, 1992·Applied and Environmental Microbiology·S BereswillK Geider
Jul 1, 1995·Applied and Environmental Microbiology·S BereswillK Geider
Dec 1, 1995·Applied and Environmental Microbiology·J J SmithG S Saddler
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Mar 1, 1997·Journal of Applied Microbiology·M T MomolH S Aldwinckle

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Citations

Jun 19, 2012·Annual Review of Phytopathology·Mickael MalnoyBrion Duffy
Mar 30, 2007·Molecular Biology of the Cell·Zhengfang Cui, Patrick J DiMario
Oct 1, 2014·The American Journal of Tropical Medicine and Hygiene·Ulises Rodriguez-PradoJose Juan Martinez-Maya

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