Characterization of estrogen-dependent growth of cultured MCF-7 human breast-cancer cells expressing 17beta-hydroxysteroid dehydrogenase type 1

International Journal of Cancer. Journal International Du Cancer
M MiettinenR Vihko

Abstract

17beta-hydroxysteroid dehydrogenase (17HSD) type I converts the weakly active estrogen, estrone, into highly active estradiol. In addition to being essential for gonadal estradiol biosynthesis, the enzyme is also expressed in a significant proportion of breast tumors. In order to study the role of the enzyme in estrogen-dependent growth of breast cancer, MCF-7 breast-cancer cells stably expressing human 17HSD type I were generated. In control MCF-7 cells a very low 17HSD activity was observed and, in line with its low estrogenic activity, estrone was devoid of the growth-enhancing effect of estradiol. The presence of the enzyme in the stably transfected MCF-7 cells resulted in a rapid conversion of estrone into estradiol but did not alter the estrogen-receptor concentration in the cells. However, in transfected cells, estrone had a growth-promoting effect practically identical to that of estradiol. The presence or absence of 17HSD type I in breast-cancer cells may therefore be decisive with regard to estrogen exposure and the estrogen-responsive growth of breast-cancer tissues.

References

Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·C MartelF Labrie
Feb 1, 1992·International Journal of Cancer. Journal International Du Cancer·M PoutanenR Vihko
Jul 1, 1991·Molecular and Cellular Endocrinology·F Labrie
Nov 1, 1986·The Journal of Clinical Endocrinology and Metabolism·A GompelP Mauvais-Jarvis
Jan 1, 1986·Breast Cancer Research and Treatment·S J SantnerR J Santen
Aug 15, 1986·International Journal of Cancer. Journal International Du Cancer·J M McNeillV H James
Jul 1, 1984·The Journal of Clinical Endocrinology and Metabolism·S J SantnerR J Santen
Dec 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·M PoutanenR Vihko

❮ Previous
Next ❯

Citations

Mar 3, 2009·Annals of the New York Academy of Sciences·Shuji NagasakiHironobu Sasano
Jun 3, 2004·Biochimica Et Biophysica Acta·Jorge R Pasqualini
Aug 26, 2006·The Journal of Steroid Biochemistry and Molecular Biology·D SongG Pelletier
Jan 13, 2006·Molecular and Cellular Endocrinology·P VihkoA Pulkka
Apr 30, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Jorge R Pasqualini, Gérard S Chetrite
Dec 1, 2010·Hormone Molecular Biology and Clinical Investigation·Tove SivikAgneta Jansson
Feb 19, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Jiong ChenSheng-Xiang Lin
Jun 21, 2005·Journal of Biomolecular Screening·Leena KokkoTero Soukka
Sep 26, 2017·The Journal of Clinical Endocrinology and Metabolism·Lorna C GilliganPaul A Foster
Dec 22, 1999·Breast Cancer Research and Treatment·M MiettinenP Vihko
Oct 16, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Trevor M Penning, Andrea J Detlefsen
Feb 13, 2001·Molecular and Cellular Endocrinology·P VihkoD Ghosh
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·H PeltoketoP Vihko
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·P VihkoV Isomaa
Mar 18, 2004·Molecular and Cellular Endocrinology·P VihkoV Isomaa

❮ Previous
Next ❯

Related Concepts