Characterization of exoskeletal proteins from the American lobster, Homarus americanus

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M NousiainenS O Andersen

Abstract

Proteins from the calcified exoskeleton of the lobster, Homarus americanus, were extracted and separated by two-dimensional gel-electrophoresis. Electroblotting the proteins onto polyvinylidene difluoride (PVDF) membranes followed by sequence determination gave 16 N-terminal amino-acid sequences and revealed that further eight proteins were N-terminally blocked. The relative molecular mass, M(r), was obtained for most of the electrophoretically separated proteins by means of matrix-assisted laser desorption mass spectrometry (MALDIMS) after electroelution from Coomassie-stained two-dimensional polyacrylamide gels. Eleven proteins were purified from extracts of the exoskeleton by low pressure ion exchange chromatography and reversed-phase high performance chromatography, and their sequences were determined by combined use of Edman degradation and mass spectrometry. Good agreement was obtained between the M(r)-values measured by mass spectrometry and those calculated from the sequences. Five of the sequenced proteins contain two copies of a previously observed 18-residue sequence motif, while a couple of the remaining sequences show similarity to sequences of exoskeletal proteins from shrimps and spiders. Only limited similarity ...Continue Reading

References

Dec 1, 1975·Comparative Biochemistry and Physiology. A, Comparative Physiology·B S Welinder
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S O Andersen
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Xu, R V Lewis
Sep 20, 1988·Journal of Molecular Biology·J E Rebers, L M Riddiford
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·A H KhanE A Sokoloski
Feb 1, 1974·Comparative Biochemistry and Physiology. A, Comparative Physiology·B S Welinder
Oct 1, 1994·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S L JacobsenP Højrup
Feb 1, 1995·Insect Biochemistry and Molecular Biology·S O AndersenP Roepstorff
Sep 1, 1996·Insect Biochemistry and Molecular Biology·T NorupP Højrup
Feb 1, 1997·Insect Biochemistry and Molecular Biology·S O AndersenP Roepstorff
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M KraghS O Andersen

❮ Previous
Next ❯

Citations

Jun 9, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anna Wynn, Thomas H Shafer
Apr 23, 2003·Biochemical and Biophysical Research Communications·Isao FukudaToshiki Watanabe
Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T WatanabeM Kono
Aug 6, 1998·Insect Biochemistry and Molecular Biology·S O Andersen
May 13, 1999·Insect Biochemistry and Molecular Biology·V A IconomidouS J Hamodrakas
Jan 26, 2000·Insect Biochemistry and Molecular Biology·S MissiosJ S Doctor
Feb 27, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S O Andersen
Jul 30, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S O Andersen
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T IkeyaT Watanabe
Mar 12, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Nicholas DitzelPeter Højrup
Oct 12, 2004·Insect Biochemistry and Molecular Biology·Toru TogawaSusumu Izumi
Jun 1, 2006·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Francie E CoblentzThomas H Shafer
Aug 27, 2002·Fish & Shellfish Immunology·Tor HaugOlaf B Styrvold
May 17, 2014·Small·Lessa Kay GrunenfelderDavid Kisailus
Dec 26, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Lindsay M Faircloth, Thomas H Shafer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M KraghS O Andersen
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
S O Andersen
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
S O Andersen
© 2022 Meta ULC. All rights reserved