Characterization of extended co-culture of non-typeable Haemophilus influenzae with primary human respiratory tissues.

Experimental Biology and Medicine
Dabin RenDayle A Daines

Abstract

Non-typeable Haemophilus influenzae (NTHi) are human-adapted Gram-negative bacteria that comprise part of the normal flora of the human upper airway, but are also responsible for a number of mucosal infections such as otitis media and bronchitis. These infections often recur and can become chronic. To characterize the effect of long-term co-culture of NTHi with human tissues, we infected primary respiratory epithelial cells grown at the air-liquid interface with three NTHi strains over a range of 1-10 days. Scanning and transmission electron microscopy of tissues confirmed that intact NTHi were persisting paracellularly, while organisms observed in intracellular vacuoles appeared degraded. Furthermore, the apical surface and tight junctions of the infected tissues were undisturbed, with high transepithelial electrical resistances, while the basal cell layer displayed more junctional disorganization and wider intercellular spaces than the uninfected control tissues. Although the tissues elaborated the cytokine profile reported for NTHi-caused otitis media in vivo, there was little change in the dynamics of cytokine secretion over the time points tested. Finally, we report that NTHi strains released outer membrane vesicles (OMVs)...Continue Reading

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Citations

Nov 6, 2012·Research in Microbiology·Ian A MacDonald, Meta J Kuehn
Sep 18, 2015·Journal of Immunology Research·João Henrique CamposAna Claudia Torrecilhas
May 16, 2015·Nature Reviews. Immunology·Maria Kaparakis-Liaskos, Richard L Ferrero
Jan 17, 2015·International Journal of Medical Microbiology : IJMM·Sandro RoierStefan Schild
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
Apr 27, 2017·FEMS Microbiology Letters·Charlotte VolgersFrank R M Stassen
Oct 17, 2012·Infection and Immunity·Forrest K RaffelKevin M Mason
Jul 26, 2018·Journal of Extracellular Vesicles·Rodrigo P SoaresAna Claudia Torrecilhas
Apr 5, 2014·Current Opinion in Infectious Diseases·Farshid Jalalvand, Kristian Riesbeck
Apr 10, 2019·Infection and Immunity·Daniel CozensRobert L Davies
Mar 17, 2021·Molecular Immunology·William J GilmoreMaria Kaparakis-Liaskos
May 23, 2021·Advanced Drug Delivery Reviews·Anna Lena JungBirke J Benedikter
May 19, 2021·Trends in Microbiology·Subhash DhitalThomas Naderer
Aug 24, 2021·Frontiers in Cellular and Infection Microbiology·Jake R WeeksTom M A Wilkinson

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
ELISArray
enzyme-linked immunosorbent assay
scanning electron microscopy
scanning
transmission electron microscopy

Software Mentioned

Magellan
SigmaStat
Discovery Workbench

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