Characterization of extracellular glucosyltransferase activity of Steptococcus mutans.

Infection and Immunity
H K Kuramitsu

Abstract

The extracellular glycosyltransferase activity of Sterptococcus mutans GS-5 has been resolved into two non-overlapping fractions after gel filtration chromatography on Bio Gel A-15 columns. The activity eluting in the void volume, fraction A, was highly aggregated and synthesized both soluble and insoluble glucans. The activity retarded by the resin, fraction B, synthesized only soluble glucan. Almost all of the extracellular glucosyltransferase activity was eluted in the void volume when the cells were grown in Todd-Hewitt medium. However, most of the activity migrated as the lower-molecular-weight species when cells were grown under conditions which inhibit insoluble glucan formation. The activities in both fractions had identical temperature and pH optima as well as similar Km values for sucrose. Fraction A synthesized both alpha-1,3- and alpha-1,6- linked glucans, whereas fraction B catalyzed alpha-1,6-glucan formation. Fraction B has been purified to near homogeneity and is also aggregated with a subunit molecular weight of 45,000. The properties of the glucosyltransferases in both fractions are discussed in terms of the role of the enzymes in both soluble and insoluble glucan formation.

References

Oct 1, 1972·Analytical Biochemistry·A H Wardi, G A Michos
Sep 1, 1973·Journal of Bacteriology·H K Kuramitsu
Oct 1, 1974·Journal of Bacteriology·G R GermaineC F Schachtele
Dec 29, 1974·Biochimica Et Biophysica Acta·M Kobayashi, K Matsuda
Apr 1, 1974·Journal of Bacteriology·A M ChludzinskiC F Schachtele
Jan 1, 1970·Caries Research·J Carlsson
Sep 24, 1971·Science·H W Scherp
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

❮ Previous
Next ❯

Citations

Oct 1, 1980·Journal of Dental Research·J J BozzolaI L Shechmeister
Aug 1, 1977·Journal of Dental Research·T J MontvilleA J Sinskey
Jan 19, 2008·Journal of Medical Microbiology·Wen-Chuan HuangJean-San Chia
May 24, 2005·Infection and Immunity·Yukie ShibataYoshihisa Yamashita
Jun 1, 1978·Carbohydrate Research·M M McCabe, E E Smith
Jan 1, 1977·Infection and Immunity·J A Beeley, P M Black
Jan 1, 1977·Infection and Immunity·D J Smith, M A Taubman
Jun 1, 1978·Infection and Immunity·H K Kuramitsu, L Ingersoll
May 1, 1991·Infection and Immunity·J S ChiaC S Yang
Sep 1, 1992·Journal of Bacteriology·Y J Nakano, H K Kuramitsu
Jan 1, 1983·Journal of Bacteriology·J P RobesonR Curtiss
Mar 18, 1982·Biochimica Et Biophysica Acta·A ShimamuraH Mukasa
Aug 1, 1983·Infection and Immunity·D PerryH K Kuramitsu
Sep 1, 1993·Infection and Immunity·T Shiroza, H K Kuramitsu
Aug 1, 1980·Infection and Immunity·H K KuramitsuM McGuinness

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.