Characterization of filament-forming CTP synthases from Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
Manuel DaumannTorsten Möhlmann

Abstract

Cytidine triphosphate (CTP) is essential for DNA, RNA and phospholipid biosynthesis. De novo synthesis is catalyzed by CTP synthases (CTPS). Arabidopsis encodes five CTPS isoforms that unanimously share conserved motifs found across kingdoms, suggesting all five are functional enzymes. Whereas CTPS1-4 are expressed throughout Arabidopsis tissues, CTPS5 reveals exclusive expression in developing embryos. CTPS activity and substrates affinities were determined for a representative plant enzyme on purified recombinant CTPS3 protein. As demonstrated in model organisms such as yeast, fruit fly and mammals, CTPS show the capacity to assemble into large filaments called cytoophidia. Transient expression of N- and C-terminal YFP-CTPS fusion proteins in Nicotiana benthamiana allowed to monitor such filament formation. Interestingly, CTPS1 and 2 always appeared as soluble proteins, whereas filaments were observed for CTPS3, 4 and 5 independent of the YFP-tag location. However, when similar constructs were expressed in Saccharomyces cerevisiae, no filaments were observed, pointing to a requirement for organism-specific factors in vivo. Indications for filament assembly were also obtained in vitro when recombinant CTPS3 protein was incubat...Continue Reading

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Citations

Oct 27, 2018·PLoS Genetics·Shanshan ZhangJi-Long Liu
May 1, 2019·International Journal of Molecular Sciences·Qing DongXi-Hong Shen
Feb 10, 2021·G3 : Genes - Genomes - Genetics·Shanshan ZhangJi-Long Liu
Apr 23, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Bo ZhangJi-Long Liu
Jun 9, 2020·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Shuang ZhouJi-Long Liu
Mar 20, 2021·Experimental Cell Research·Qiao-Qi WangJi-Long Liu
Mar 17, 2021·G3 : Genes - Genomes - Genetics·Bo ZhangJi-Long Liu
Apr 5, 2021·Experimental Cell Research·Han-Chao FengJi-Long Liu
May 4, 2021·Frontiers in Plant Science·Daniel HicklTorsten Möhlmann
Jun 1, 2021·Plant Biotechnology Journal·Jinmi YoonGynheung An
Jun 16, 2021·Experimental Cell Research·Chia-Chun ChangJi-Long Liu
Jul 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xian ZhouJi-Long Liu
Jul 11, 2021·Journal of Experimental Botany·Daniel HicklTorsten Möhlmann
Dec 18, 2021·Frontiers in Plant Science·Leo BellinTorsten Möhlmann

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