PMID: 2904638Aug 1, 1987

Characterization of fimbriae from Bacteroides fragilis

Microbial Pathogenesis
J van DoornD M MacLaren

Abstract

Fimbriae derived from Bacteroides fragilis strain BE1 (BE1 fimbriae) appeared to be composed of subunits with a molecular weight of 40,000. Under the electron microscope the fimbriae could be visualized as straight filaments with a diameter of 4 nm. It appeared that production of the BE1 fimbriae is repressed under conditions of iron limitation, and at a growth temperature of 20 degrees C. Antibodies raised against the 40,000 dalton polypeptide, purified by means of preparative SDS-polyacrylamide gelelectrophoresis, recognized the native fimbriae, as was shown by immunogold labelling of intact bacterial cells, and by immunoprecipitation. Immunoblot experiments showed that other strains of B fragilis tested produced polypeptides, ranging in molecular weight from 40,000 to 42,000, that are antigenically related to the BE1 fimbrial subunit. No haemagglutination activity could be associated with the BE1 fimbriae.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1986·Journal of Medical Microbiology·W A VelD M MacLaren
Aug 1, 1985·European Journal of Clinical Microbiology·A M Verweij-Van VughtD M MacLaren
May 23, 1974·The New England Journal of Medicine·S L Gorbach, J G Bartlett
Nov 1, 1969·Archives of Biochemistry and Biophysics·M Steinbuch, R Audran
Jun 1, 1984·Journal of General Microbiology·J E BeesleyC M Thorley
Jan 1, 1984·Antonie van Leeuwenhoek·D M MacLarenJ A Kaan
Mar 1, 1984·Reviews of Infectious Diseases·T Hofstad

Citations

Nov 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T Hofstad
Oct 1, 1992·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·R M DominguesM C Ferreira
Apr 12, 2016·Cell·Qingping XuIan A Wilson
Apr 23, 2016·Cell·Michael J Coyne, Laurie E Comstock
Apr 1, 1988·Microbial Pathogenesis·A A PotterJ Gilchrist

Related Concepts

Antigens, Bacterial
Bacterial Adhesion
Bacterial Proteins
Bacteroides fragilis
Isohemagglutinins
Immunocytochemistry
Electron Microscopy
Bacterial Pilus

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