PMID: 6172152Dec 28, 1981Paper

Characterization of five members of the actin gene family in the sea urchin

Biochimica Et Biophysica Acta
P A OverbeekL J Kleinsmith

Abstract

Hybridization of an actin cDNA clone (pSA38) to restriction enzyme digests of Strongylocentrotus purpuratus DNA indicates that the sea urchin genome contains at least five different actin genes. A sea urchin genomic clone library was screened for recombinants which hydridize to pSA38 and four genomic clones were isolated. Restriction maps were generated which indicate that three of these recombinants contain different actin genes, and that the fourth may be an allele to one of these. The restriction maps suggest that one clone contains two linked actin genes. This fact, which was confirmed by heteroduplex analysis, indicates that the actin gene family may be clustered. The linked genes are oriented in the same direction and spaced about 8.0 kilobases apart. In heteroduplexes between genomic clones two intervening sequences were seen. Significant homology is confined to the actin coding region and does not include any flanking sequence. Southern blot analysis reveals that repetitive DNA sequences are found in the region of the actin genes.

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Citations

Jul 30, 1981·Developmental Biology·G T MerlinoL J Kleinsmith
Jun 1, 1989·Developmental Biology·P LuValleB R Olsen
Jan 1, 1994·Matrix Biology : Journal of the International Society for Matrix Biology·I RokosB R Olsen
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Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D McCormickB R Olsen
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M K GordonB R Olsen
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y MuragakiB R Olsen
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Oct 1, 1983·Molecular and Cellular Biology·P J JohnsonG P Moore

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