PMID: 2110535Apr 24, 1990Paper

Characterization of four G0-type proteins purified from bovine brain membranes

FEBS Letters
A InanobeT Katada

Abstract

Recently we reported there were at least four types of G0 or G0-like proteins in bovine brain membranes based on their elution profiles from Mono Q columns and their immunological reactivities; one of the proteins was purified as an alpha-monomeric form, and the others as alpha beta gamma-trimers. The four proteins, of which alpha-subunits were confirmed to be a family of G0-type by an immunoblot analysis, were thus referred to as alpha (0)1, G(0)2, G(0)3 and G(0)4, respectively, in order of their elutions from the column. Immunostained peptide mappings arising from proteolytic digestions of the four alpha-subunits, together with their fragmentation patterns containing radiolabeled ADP-ribose that had been incorporated by pertussis toxin-catalyzed ADP-ribosylation, suggested that the four G0-alpha were classified into either of two groups such as alpha (0)1 and G(0)2-alpha, or G(0)3-alpha and G(0)4-alpha. The kinetic parameters of their GTPase activities, however, revealed that there were different properties between alpha (0)1 and G(0)2-alpha or G(0)3-alpha and G(0)4-alpha. Thus, the four G0-type proteins appeared to be different entities from one another.

References

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H ItohK Suzuki
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann

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Citations

Dec 15, 1994·European Journal of Pharmacology·R ZumbihlB Rouot
Apr 8, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G G Holz, T J Turner
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B D Carter, F Medzihradsky
Aug 1, 1994·Journal of Neuroendocrinology·J C PainsonR Collu
Mar 15, 1992·European Journal of Biochemistry·H HeithierE J Helmreich
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ExnerB Nürnberg
Mar 3, 1995·The Journal of Biological Chemistry·M D WilcoxJ D Hildebrandt
May 1, 1992·Archives of Biochemistry and Biophysics·K KontaniT Katada
Mar 15, 1991·Biochemical and Biophysical Research Communications·K SpicherW Rosenthal

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