Characterization of fourteen strains of Neisseria gonorrhoeae: structural analyses and serum reactivities

Molecular Microbiology
R K PettitR C Judd

Abstract

Resistance to normal human serum (NHS) killing in Neisseria gonorrhoeae has been associated with particular types of Protein I (PI) and lipopolysaccharide (LPS), but many exceptions exist, and the role of these structures in determining serum reactivities remains controversial. In reality, the response of the gonococcus to NHS is probably governed by several parameters involving a number of outer-membrane (OM) components. We surveyed the serum reactivities of 14 strains of N. gonorrhoeae and characterized each of their major OM components. The strains presented a spectrum of sensitivity to pooled NHS. As assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, immunoblotting, and peptide mapping, the strains were also quite heterogeneous in terms of PI, H.8 antigen, and LPS type, and the presence of the 2-1-L8 epitope. Five of the strains had identical PIAs in varying LPS and H.8 backgrounds, and four had identical PIBs in varying LPS and H.8 backgrounds. As assessed by electrophoretic migration and monoclonal antibody binding, Protein III and the 44,000 Dalton protein were identical in these strains. We found no association between PI subclass and serum sensitivity, while H.8 and LPS variation appeared to be rel...Continue Reading

References

Feb 1, 1986·The Journal of Infectious Diseases·P F SparlingM So
Nov 1, 1985·The Journal of Clinical Investigation·K A JoinerP A Rice
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Feb 1, 1984·The Journal of Infectious Diseases·W M ShaferP F Sparling
Mar 1, 1980·Journal of Bacteriology·R L McDade, K H Johnston
May 1, 1981·Infection and Immunity·J G CannonP F Sparling
Jan 1, 1982·The Journal of Infectious Diseases·J F JamesG F Brooks
Dec 1, 1981·Infection and Immunity·J Swanson
Jan 1, 1982·Journal of General Microbiology·H SchneiderG B Pier
Jun 1, 1982·Infection and Immunity·M R TamR C Nowinski

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