Characterization of fsr, a regulator controlling expression of gelatinase and serine protease in Enterococcus faecalis OG1RF

Journal of Bacteriology
X QinB E Murray

Abstract

We have previously identified a locus, fsr, a homologue of staphylococcal agr loci, which positively regulates the expression of gelatinase and serine protease (encoded by gelE and sprE, respectively) in Enterococcus faecalis OG1RF. The expression of the three genes in the fsr locus, fsrA, fsrB, and fsrC, appears to be autoregulated, and we have shown that mutants with insertion disruptions in each of these three genes were significantly attenuated in a mouse peritonitis model compared to the parent strain. In the present study, we showed that fsrB and fsrC are highly expressed in the postexponential growth phase and that their expression is cell density dependent. Reverse transcriptase PCR using primers covering the intergenic regions in the fsr/gelE loci confirmed that fsrB and fsrC, as well as gelE and sprE, are cotranscribed. We also showed, using a nonpolar fsrB deletion mutant, that fsrB, the homologue of agrB of staphylococci with unknown function, is required for the regulatory function of fsr. Primer extension and analysis of transcriptional fusions indicated the presence of promoters immediately upstream of fsrA, of fsrB, and of gelE and that the fsrB and gelE promoters are fsr dependent, while the fsrA promoter is an...Continue Reading

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Citations

Sep 27, 2002·Current Opinion in Pharmacology·Keith Stephenson, James A Hoch
Aug 4, 2012·Journal of Proteome Research·Changlin ShaoJing Yuan
Feb 16, 2012·The Journal of Biological Chemistry·Jennifer R MastroianniAndré J Ouellette
Nov 19, 2008·Antimicrobial Agents and Chemotherapy·Jiro NakayamaKenji Sonomoto
Aug 7, 2007·Applied and Environmental Microbiology·Aurélie RieuJean Guzzo
Nov 26, 2010·Applied and Environmental Microbiology·Jessica R Galloway-PeñaBarbara E Murray
Apr 23, 2008·Infection and Immunity·Eliette Riboulet-BissonJean-Christophe Giard
Jun 17, 2009·Infection and Immunity·Pascale S GuitonScott J Hultgren
Nov 2, 2012·Infection and Immunity·Melissa R CruzDanielle A Garsin
Aug 18, 2010·Infection and Immunity·Lance R ThurlowLynn E Hancock
May 4, 2011·Infection and Immunity·Charlotte MichauxJean-Christophe Giard
Mar 6, 2013·Infection and Immunity·Kristi L FrankGary M Dunny
Mar 10, 2009·Journal of Bacteriology·Christine M SedgleySusan E Flannagan
Oct 9, 2007·Journal of Bacteriology·Maria Florencia Del PapaMarta Perego
Jan 25, 2011·Journal of Bacteriology·María Florencia Del Papa, Marta Perego
Nov 15, 2011·Journal of Bacteriology·Vijayalakshmi S Iyer, Lynn E Hancock
Oct 11, 2002·Journal of Bacteriology·Lynn Hancock, Marta Perego
Dec 18, 2003·Journal of Bacteriology·Christopher J KristichGary M Dunny
Jun 29, 2002·Journal of Clinical Microbiology·S K PillaiR T Inouye
Aug 9, 2003·Journal of Clinical Microbiology·Ronald N Jones, Lalitagauri M Deshpande

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