Characterization of functional antibody and memory B-cell responses to pH1N1 monovalent vaccine in HIV-infected children and youth

PloS One
Donna J CurtisAdriana Weinberg

Abstract

We investigated immune determinants of antibody responses and B-cell memory to pH1N1 vaccine in HIV-infected children. Ninety subjects 4 to <25 years of age received two double doses of pH1N1 vaccine. Serum and cells were frozen at baseline, after each vaccination, and at 28 weeks post-immunization. Hemagglutination inhibition (HAI) titers, avidity indices (AI), B-cell subsets, and pH1N1 IgG and IgA antigen secreting cells (ASC) were measured at baseline and after each vaccination. Neutralizing antibodies and pH1N1-specific Th1, Th2 and Tfh cytokines were measured at baseline and post-dose 1. At entry, 26 (29%) subjects had pH1N1 protective HAI titers (≥1:40). pH1N1-specific HAI, neutralizing titers, AI, IgG ASC, IL-2 and IL-4 increased in response to vaccination (p<0.05), but IgA ASC, IL-5, IL-13, IL-21, IFNγ and B-cell subsets did not change. Subjects with baseline HAI ≥1:40 had significantly greater increases in IgG ASC and AI after immunization compared with those with HAI <1:40. Neutralizing titers and AI after vaccination increased with older age. High pH1N1 HAI responses were associated with increased IgG ASC, IFNγ, IL-2, microneutralizion titers, and AI. Microneutralization titers after vaccination increased with high I...Continue Reading

References

Apr 1, 1989·Journal of Medical Virology·K Hedman, S A Rousseau
Aug 11, 1989·JAMA : the Journal of the American Medical Association·P G MiottiM L Clements
Mar 1, 1994·The Pediatric Infectious Disease Journal·E G ChadwickK M Edwards
Jul 29, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·S A MadhiK P Klugman
May 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A D FineK Fukuda
Jul 15, 2006·PLoS Pathogens·Giovanna RappoccioloCharles R Rinaldo
Dec 1, 2006·Molecular Immunology·Muriel ViauMoncef Zouali
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jason HoAnthony S Fauci
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie HartPeter Kelleher
Jun 12, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Alessandra ViganòMario Clerici
Jun 28, 2008·The Journal of Infectious Diseases·Marianne C JacobsenHelen E Baxendale
Mar 26, 2009·Nature Reviews. Immunology·Susan Moir, Anthony S Fauci
Jun 29, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Adriana WeinbergUNKNOWN IMPAACT P1057 Team
Sep 28, 2010·Blood·Julie FontaineUNKNOWN Montreal Primary HIV Infection and Long-Term Non-Progressor Study Groups
Dec 22, 2010·Paediatric Respiratory Reviews·Christine JonesBeate Kampmann
May 3, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Suresh PallikkuthSavita Pahwa
Jul 15, 2011·The Journal of Allergy and Clinical Immunology·Suresh PallikkuthSavita Pahwa
Nov 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chao QiuJianqing Xu
May 4, 2012·The Journal of Infectious Diseases·Gabriel K PedersenRebecca J Cox
May 31, 2012·Vaccine·Rogier BodewesGuus F Rimmelzwaan
Jan 25, 2013·AIDS·Simone PensierosoGabriella Scarlatti

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Methods Mentioned

BETA
blood draws
ELISA
flow cytometry

Clinical Trials Mentioned

NCT00992836

Software Mentioned

SAS
Bio
R
Plex manager
PRISM

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