Abstract
Although accumulating evidence within the past 5 years strongly supports the importance of platelet P2X(1) receptors in hemostasis and thrombosis, P2X(1) receptors of platelet and/or its progenitor cell, megakaryocyte, have not been fully characterized. The aim of this study was to electrophysiologically and pharmacologically characterize the functional P2X(1) receptors on mouse megakaryocytes. The currents in response to nucleotides were examined using the patch-clamp whole-cell recording. The agonist profile of megakaryocyte P2X(1) receptors was ATP>alpha,beta-methylene ATP>beta,gamma-methylene ATP. The P2X(1) receptors exhibited substantial monovalent as well as divalent cation permeability and the ratios of Na(+) to Cs(+) and Ca(2+) to Cs(+) permeability were 1 and 2.5, respectively. P2X receptor antagonists except suramin significantly inhibited the P2X(1) responses with an IC(50) values of 0.4 microM for pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS), 0.3 microM for 2',3'-O-(2,4,6-trinitophenyl)-adenosine 5'-triphosphate (TNP-ATP), 20 microM for reactive blue 2 (RB2), or 160 microM for 8,8'-(carbonylbis(imino-3,1-phenylene carbonylimino)bis(1,3,5-naphthalenetrisulfonic acid) (NF023), respectively. Suramin had n...Continue Reading
References
Feb 1, 1992·The Journal of Physiology·M IkedaY Maruyama
Dec 1, 1990·The Journal of Physiology·K Kawa
Jul 16, 1987·Nature·C D Benham, R W Tsien
Oct 1, 1987·The Journal of Physiology·Y Maruyama
Jan 1, 1994·British Journal of Pharmacology·H UneyamaN Akaike
Oct 15, 1994·The Journal of Physiology·B Somasundaram, M P Mahaut-Smith
Jun 1, 1994·Pflügers Archiv : European journal of physiology·M IkedaY Maruyama
Oct 1, 1993·The Journal of Physiology·C UneyamaN Akaike
Feb 9, 1996·The Journal of Biological Chemistry·A B MacKenzieS O Sage
Sep 1, 1996·The Journal of Physiology·K Kawa
Feb 17, 1998·Biochemical and Biophysical Research Communications·T J ScaseR J Evans
Jun 13, 1998·The Journal of Biological Chemistry·B SunJ Kambayashi
Sep 2, 1998·British Journal of Pharmacology·C J LewisR J Evans
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·R A North, A Surprenant
Aug 26, 2000·British Journal of Pharmacology·M P Mahaut-SmithR J Evans
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·G Lambrecht
Jan 4, 2001·Biochemical Pharmacology·M Ikeda, Y Maruyama
Aug 25, 2001·Naunyn-Schmiedeberg's Archives of Pharmacology·K BraunG Lambrecht
Jan 30, 2002·British Journal of Pharmacology·Catherine VialRichard J Evans
Aug 15, 2002·The Journal of Biological Chemistry·Masahiro IkedaMasato Yasui
Sep 19, 2002·Molecular Pharmacology·Scott S WildmanBrian F King
Sep 25, 2002·Physiological Reviews·R Alan North
Jan 11, 2003·Blood·Cécile OuryMarc F Hoylaerts
Apr 12, 2003·Current Opinion in Pharmacology·Satya P KunapuliTodd M Quinton
Aug 13, 2003·The Journal of Experimental Medicine·Béatrice HechlerChristian Gachet
Jun 19, 2004·Platelets·Martyn P Mahaut-SmithRichard J Evans
Mar 29, 2005·The Journal of Pharmacology and Experimental Therapeutics·Béatrice HechlerChristian Gachet
May 26, 2005·Blood·Gwen TolhurstMartyn P Mahaut-Smith
Sep 15, 2005·Naunyn-Schmiedeberg's Archives of Pharmacology·Susanne HornerGünter Lambrecht
Citations
Feb 20, 2008·The Journal of Experimental Medicine·Craig N MorrellCharles J Lowenstein
Sep 4, 2008·The Journal of Biological Chemistry·Joan A SimR Alan North
Oct 16, 2007·British Journal of Pharmacology·J A SimR A North
Aug 12, 2015·Purinergic Signalling·Geoffrey Burnstock
Jul 28, 2010·Blood·Tobias WoehrleWolfgang G Junger
Apr 21, 2007·Stem Cells·Elisabetta CoppiFelicita Pedata
Jan 1, 2010·American Journal of Physiology. Cell Physiology·José P YoungAdam Myers
Jul 27, 2018·Research and Practice in Thrombosis and Haemostasis·Tania KamalMaggie L Kalev-Zylinska