PMID: 8592193Nov 1, 1995Paper

Characterization of GABA current in rat septal cholinergic neurons in culture and its modulation by metal cations

Journal of Neurophysiology
E Kumamoto, Y Murata

Abstract

1. With the aim of characterizing the functional and pharmacological properties of gamma-aminobutyric acid-receptor (type A; GABAAR) channels expressed in a given type of brain neurons, we obtained whole cell voltage-clamp recordings from rat septal cholinergic neurons in primary culture. 2. GABA-induced currents (IGABAS) were accompanied by an increase in conductance and reversed the direction at the Cl- equilibrium potential. The permeability sequence of GABA-activated Cl- channel for various anions (ratio to Cl- permeability) was found to be SCN- (1.72) > or = I- (1.60) > or = Br- (1.55) > Cl- (1) > HCO2- (0.42) > F- (0). 3. GABA applied by local perfusion produced an IGABA that enhanced in amplitude with increasing concentrations (EC50 = 21.4 microM; nH = 1.70) and desensitized at higher concentrations. Bicuculline inhibited IGABA (20 microM) competitively (IC50 = 0.963 microM) with a dissociation constant of approximately 0.5 microM. Picrotoxin depressed IGABA (20 microM) noncompetitively in a dose- and use-dependent manner (IC50: approximately 100 microM for peak IGABA). 4. IGABA was potentiated by diazepam at 1 and 10 microM (by 20% at 1 microM for IGABA evoked at 10 microM) but not at 0.1 and 100 microM. IGABA was facil...Continue Reading

Citations

Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·F Le FollL Cazin
Oct 1, 2018·The European Journal of Neuroscience·Yukihiro Yamada, Rebecca A Prosser
Sep 17, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Carla Marchetti
Oct 24, 2018·Cell Communication and Signaling : CCS·Julianna KardosKatalin Jemnitz
Jan 1, 1997·Neurobiology of Disease·M P Cuajungco, G J Lees
Jun 1, 1997·Progress in Neurobiology·E Kumamoto

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