Characterization of gag and pol sequences from long-term survivors of human immunodeficiency virus type 1 infection

Virology
Y HuangD D Ho

Abstract

We previously identified a group of 10 long-term survivors (LTS) of human immunodeficiency virus type 1 (HIV-1) infection. Extensive biological analysis revealed that some of these individuals do well, at least in part, because they possess weakened or attenuated viruses. Also, previously, to determine the genotype associated with the attenuated phenotype in vivo, we characterized nef, vif, vpr, vpu, env, and LTR in our cohort of LTS. In this study, we analyzed gag and pol genes derived from eight individuals in our cohort. For each subject multiple full-length gag and pol clones were obtained for analysis. In most cases, the sequences derived from the LTS had an intact open reading frame. At the protein level, there were no discernible differences between the sequences derived from LTS and those derived from patients with AIDS. Thus, no common defect in gag and pol was found in our cohort. One individual (subject SF), however, had only Gag-defective proviral sequences (10 of 10) in his peripheral blood mononuclear cells. Furthermore, longitudinal studies of the samples collected from SF over a 2-year period showed that all p17 gag clones sequenced (24 of 24) were defective due to G-to-A hypermutations. This viral defect in Gag...Continue Reading

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Citations

Jan 8, 2004·AIDS Research and Human Retroviruses·Irene N KoulinskaBoris Renjifo
Nov 10, 2009·AIDS Research and Human Retroviruses·D B TzitzivacosM A Papathanasopoulos
Mar 22, 2007·Retrovirology·Xia JinHarold Smith
Jan 14, 2005·Journal of Medical Virology·Angsana PhuphuakratPrasert Auewarakul
Aug 1, 2006·The Journal of Infection·Isabelle MaletAnne-Geneviève Marcelin
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Jul 20, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ruengpung SutthentPongsakdi Chaisilwatana

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