PMID: 9549105Apr 29, 1998Paper

Characterization of galactosidases from Aspergillus niger: purification of a novel alpha-galactosidase activity

Enzyme and Microbial Technology
P ManzanaresJ Visser

Abstract

An enzyme with beta-galactosidase activity and three proteins exhibiting alpha-galactosidase activity were purified from a culture filtrate of Aspergillus niger grown on arabinoxylan. beta-galactosidase, optimally active at pH 4 and 60-65 degrees C, was active against p-nitrophenyl-beta-D-galactopyranoside, lactose, and pectic galactan. It was not able to release galactose from sugar beet pectin or lemon pectin. Its action on pectic galactan was increased by the presence of beta-galactanase. The three forms of alpha-galactosidase activity that showed different molecular masses and pIs were found to have the same mass after deglycosylation with N-glycanase F and to be the same protein based on their N-terminal amino acid sequence data. The purified alpha-galactosidase was shown to be different from alpha-galactosidase A from A. niger. This confirmed the existence of at least two different alpha-galactosidases in A. niger. alpha-Galactosidase, optimally active at pH 4.5 and 50-55 degrees C, was active toward p-nitrophenyl-alpha-D-galactopyranoside, melibiose, raffinose, stachyose, and locust bean gum, on which substrate it exhibited synergism with beta-mannanase.

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Citations

Oct 7, 2009·Applied Microbiology and Biotechnology·Shane O'Connell, Gary Walsh
May 15, 2013·Applied Microbiology and Biotechnology·Tatsuji Sakamoto, Megumi Ishimaru
Feb 19, 2011·Applied Biochemistry and Biotechnology·Joana Gasperazzo FerreiraSebastião Tavares de Rezende
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Apr 20, 2014·Biotechnology Research International·Xiaoli LiuMichel Casgrain
Feb 7, 2012·Biotechnology Advances·Feng PengRun-Cang Sun
Oct 2, 2008·Biotechnology and Bioengineering·Liat ChillJoseph Shiloach
May 23, 2014·Biotechnology Progress·Frederico MagalhesLucília Domingues
Aug 14, 2013·Critical Reviews in Biotechnology·Priti KatroliaZhengqiang Jiang
May 7, 2019·Frontiers in Microbiology·Antonio Benítez-CabelloFrancisco Noé Arroyo-López
Apr 12, 2020·International Journal of Biological Macromolecules·C H VidyaSridevi Annapurna Singh
Feb 16, 2006·Journal of Agricultural and Food Chemistry·Danfeng Song, Sam K C Chang

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