Characterization of gamma-tubulin complexes in Aspergillus nidulans and detection of putative gamma-tubulin interacting proteins

Cell Motility and the Cytoskeleton
T AkashiBerl Oakley

Abstract

gamma-Tubulin is central to the nucleation of microtubule assembly in vivo. Although it is most obviously located at microtubule organizing centers, it is also found in soluble cytoplasmic complexes. Characterizing these complexes and identifying proteins that interact with gamma-tubulin in vivo will be necessary if gamma-tubulin function is to be understood fully. We have begun to investigate soluble complexes of gamma-tubulin in Aspergillus nidulans, the organism in which gamma-tubulin was discovered and in which a great deal of genetic and molecular genetic analysis of gamma-tubulin has been carried out. We find that approximately 32% of the gamma-tubulin in A. nidulans is soluble. Sucrose density gradients revealed that the soluble gamma-tubulin is in 8-20S complexes with little or no monomeric gamma-tubulin present. In the presence of 0.5 M KCl the average size of the complexes decreased and a peak was present between 4S and 11S. Cross-linking experiments with a zero-length cross-linker suggest that gamma-tubulin in isolated nuclei and in intact hyphae interacts physically with three proteins with molecular weights of approximately 105, 95, and 80 kDa.

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Citations

Mar 15, 2000·The Plant Cell·P BinarováP Dráber
Oct 29, 2009·Journal of Cell Science·Yi Xiong, Berl R Oakley
Jun 26, 2004·Cell Structure and Function·B R Oakley, Y N Akkari
Feb 16, 2006·The Journal of Cell Biology·Christel VérolletBrigitte Raynaud-Messina
Aug 26, 1998·European Journal of Cell Biology·R GräfM Schliwa
Jun 22, 2010·Journal of Cellular Biochemistry·Nisha E ThomasSuparna Sengupta
Dec 14, 2005·Toxicology and Industrial Health·Patricia Mussali-GalanteTeresa I Fortoul
Feb 27, 1999·Current Opinion in Cell Biology·W ZimmermanS J Doxsey
May 14, 1999·Current Opinion in Structural Biology·C Wiese, Y Zheng
May 23, 2002·European Journal of Cell Biology·Christine Daunderer, Ralph O Gräf
Oct 14, 2006·Journal of Cell Science·Christiane Wiese, Yixian Zheng

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