PMID: 7521131Aug 1, 1994Paper

Characterization of gap junctions between pairs of Leydig cells from mouse testis

The American Journal of Physiology
E M Pérez-ArmendarizA P Moreno

Abstract

Leydig cells are coupled in vivo by numerous gap junctions. In vivo and in vitro cells were immunolabeled by connexin 43 (Cx43) but not by Cx26 or Cx32 antibodies; immunoblotting confirmed specificity of Cx43 labeling. Pairs of Leydig cells dissociated from mouse testis were studied by dual whole cell voltage clamp, and a high incidence of dye (n = 20) and electrical coupling (n = 60; > 90%) was found. Coupling coefficients were near 1 and junctional conductance (gj) averaged 7.2 +/- 1.2 nS (SE, n = 40). Large transjunctional voltage (Vj) decreased gj; currents decayed exponentially with time constants of seconds that decreased at greater Vj. The residual conductance at large Vj was at least approximately 40% of the initial conductance. Exposure of cell pairs to saline solutions saturated with CO2 (n = 15) or containing 2 mM halothane (n = 15) or 3.5 mM heptanol (n = 15) rapidly and reversibly reduced gj. In eight cell pairs, gating of single junctional channels was observed during halothane-induced reduction in gj. Most gating events at Vj < 40 mV were fit by a Gaussian distribution with a mean of approximately 100 pS. With Vj > 40 mV, smaller transitions of approximately 30 pS were also recorded, and the frequency and duratio...Continue Reading

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Citations

Oct 12, 2001·The Anatomical Record·E M Pérez-ArmendarizJ F Bravo Moreno
Mar 12, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·E Martha Pérez-ArmendarizIrma Villalpando
Jun 11, 2008·Histochemistry and Cell Biology·Christina RüttingerRalph Brehm
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Georges PointisDominique Segretain
May 15, 1996·European Journal of Biochemistry·R BruzzoneD L Paul
Dec 31, 2004·American Journal of Physiology. Endocrinology and Metabolism·Alonso P MorenoE Martha Pérez-Armendariz
Feb 3, 2007·Zygote : the Biology of Gametes and Early Embryos·G IzzoS Minucci
Oct 22, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R-Marc PelletierMaría L Vitale
Feb 28, 2009·Journal of Anatomy·Yong-Guang GongRen-Shan Ge
May 12, 2004·Journal of Cellular Physiology·X DecrouyD Segretain
Sep 3, 2009·Microscopy Research and Technique·María Leiza VitaleR-Marc Pelletier
Nov 26, 2003·Molecular Carcinogenesis·Dominique SegretainGeorges Pointis
Mar 1, 1996·Pflügers Archiv : European journal of physiology·K Banach, R Weingart
Jan 11, 2002·Journal of Andrology·Angela M SuburoJuan H Tramezzani
Aug 22, 2001·Biology of Reproduction·W A RoscoeG M Kidder
Mar 6, 2013·Canadian Journal of Physiology and Pharmacology·Dan LiGerald M Kidder
Apr 20, 2000·Biochimica Et Biophysica Acta·J M CristanchoW A Varanda
Nov 2, 2005·Biochimica Et Biophysica Acta·Georges PointisDominique Segretain
Dec 20, 2005·Biochimica Et Biophysica Acta·Laetitia MichonPaolo Meda
Aug 2, 2005·Trends in Endocrinology and Metabolism : TEM·Georges Pointis, Dominique Segretain
Jun 27, 2007·The American Journal of Pathology·Ralph BrehmMartin Bergmann

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