Characterization of genes involved in the initial reactions of 4-chloronitrobenzene degradation in Pseudomonas putida ZWL73

Applied Microbiology and Biotechnology
Yi XiaoNing-Yi Zhou

Abstract

The genes encoding enzymes involved in the initial reactions during degradation of 4-chloronitrobenzene (4CNB) were characterized from the 4CNB utilizer Pseudomonas putida ZWL73, in which a partial reductive pathway was adopted. A DNA fragment containing genes coding for chloronitrobenzene nitroreductase (CnbA) and hydroxylaminobenzene mutase (CnbB) were PCR-amplified and subsequently sequenced. These two genes were actively expressed in Escherichia coli, and recombinant E. coli cells catalyzed the conversion of 4CNB to 2-amino-5-chlorophenol, which is the ring-cleavage substrate in the degradation of 4CNB. Phylogenetic analyses on sequences of chloronitrobenzene nitroreductase and hydroxylaminobenzene mutase revealed that these two enzymes are closely related to the functionally identified nitrobenzene nitroreductase and hydroxylaminobenzene mutase from Pseudomonas strains JS45 and HS12. The nitroreductase from strain ZWL73 showed a higher specific activity toward 4CNB than nitrobenzene (approximately at a ratio of 1.6:1 for the recombinant or 2:1 for the wild type), which is in contrast to the case where the nitroreductase from nitrobenzene utilizers Pseudomonas pseudoalcaligenes JS45 with an apparently lower specific activit...Continue Reading

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Citations

May 29, 2010·Applied Microbiology and Biotechnology·Ying YinNing-Yi Zhou
Feb 15, 2012·Applied Microbiology and Biotechnology·Pankaj Kumar AroraCh Venkata Ramana
Apr 21, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Joane Kathelen RustiguelMaria Cristina Nonato
Aug 2, 2011·Applied and Environmental Microbiology·Dhan PrakashB N Tiwary
Mar 22, 2008·FEMS Microbiology Reviews·María Dolores RoldánConrado Moreno-Vivián
Dec 26, 2008·Environmental Pollution·Gui-Lan NiuNing-Yi Zhou
Jul 26, 2017·ACS Sensors·Shenzheng LuoMarkita P Landry
Jun 3, 2021·Biology·Carol Thomas, Christopher D Gwenin
May 1, 2009·Journal of Environmental Sciences (China)·Haizhen WuShizhong Liang

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