Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing

PloS One
Bruce R SoutheyS L Rodriguez-Zas

Abstract

Among forager honey bees, scouts seek new resources and return to the colony, enlisting recruits to collect these resources. Differentially expressed genes between these behaviors and genetic variability in scouting phenotypes have been reported. Whole-genome sequencing of 44 Apis mellifera scouts and recruits was undertaken to detect variants and further understand the genetic architecture underlying the behavioral differences between scouts and recruits. The median coverage depth in recruits and scouts was 10.01 and 10.7 X, respectively. Representation of bacterial species among the unmapped reads reflected a more diverse microbiome in scouts than recruits. Overall, 1,412,705 polymorphic positions were analyzed for associations with scouting behavior, and 212 significant (p-value < 0.0001) associations with scouting corresponding to 137 positions were detected. Most frequent putative transcription factor binding sites proximal to significant variants included Broad-complex 4, Broad-complex 1, Hunchback, and CF2-II. Three variants associated with scouting were located within coding regions of ncRNAs including one codon change (LOC102653644) and 2 frameshift indels (LOC102654879 and LOC102655256). Significant variants were also...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Jun 25, 2004·Nucleic Acids Research·Albin SandelinBoris Lenhard
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Zhe HanEric N Olson
Jun 7, 2005·Mass Spectrometry Reviews·Amanda B HummonJonathan V Sweedler
Aug 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yasuyuki ArakaneKarl J Kramer
Sep 15, 2006·BMC Genomics·Sandra L Rodriguez-ZasGene E Robinson
Oct 27, 2006·Genome Research·Martin BeyeRobert E Page
Oct 31, 2006·Nature·UNKNOWN Honeybee Genome Sequencing Consortium
Dec 15, 2006·Molecular and Cellular Neurosciences·Kunihiro ShiomiToshinobu Yaginuma
May 29, 2007·Development Genes and Evolution·Rebecca F Spokony, Linda L Restifo
Sep 20, 2008·Nature Reviews. Genetics·Chris R SmithGene E Robinson
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Axel BrockmannJonathan V Sweedler
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Aug 1, 2009·Behavioural Brain Research·Jennifer M Tsuruda, Robert E Page
Sep 10, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wenfu MaoMay R Berenbaum
Feb 27, 2010·Insect Biochemistry and Molecular Biology·Moysés Elias-NetoMárcia M G Bitondi
Mar 10, 2010·The Journal of Heredity·Emily R MeznarOlav Rueppell
Jul 17, 2010·Journal of Theoretical Biology·Peder A Tyvand, Steinar Thorvaldsen
Jul 20, 2010·Behavior Genetics·F Bernhard KrausRobin F A Moritz
Feb 5, 2011·Nature Protocols·Geraldine M ClarkeKrina T Zondervan
May 28, 2011·Archives of Insect Biochemistry and Physiology·Xiaoli YuBaohua Xu
Jul 2, 2011·Journal of Insect Physiology·Luciana Karen CalábriaFoued Salmen Espindola
Feb 7, 2012·Journal of Biological Rhythms·Sandra L Rodriguez-ZasGuy Bloch
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Mar 10, 2012·Science·Zhengzheng S LiangGene E Robinson
Sep 1, 2012·Annual Review of Genetics·Robert E PageGro V Amdam
Dec 26, 2012·Advances in Biological Regulation·Becky Tu-SekineDaniel M Raben
Apr 16, 2013·Bioinformatics and Biology Insights·Riccardo Percudani
Oct 2, 2013·BMC Developmental Biology·Shuji UedaYasuhito Shirai
Apr 29, 2014·Journal of Insect Physiology·Jason T Vance, Stephen P Roberts
Oct 24, 2014·Annual Review of Entomology·Hua YanDanny Reinberg
Nov 22, 2014·Nucleic Acids Research·Dennis A BensonEric W Sayers

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Citations

May 17, 2017·Journal of Personality·Brent W Roberts

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Methods Mentioned

BETA
Protein Precipitation
Precipitation
chip
genotyping

Software Mentioned

Samtools
Bowtie2
BLASTN
ConSite
SNPeff tool

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