Characterization of GhRac1 GTPase expressed in developing cotton (Gossypium hirsutum L.) fibers

Biochimica Et Biophysica Acta
Hee Jin Kim, Barbara Triplett

Abstract

Cytoskeleton assembly plays an important role in determining cotton fiber cell length and morphology and is developmentally regulated. As in other plant cells, it is not clear how cytoskeletal assembly in fibers is regulated. Recently, several Rac/Rop GTPases in Arabidopsis were shown to regulate isotropic and polar cell growth of root hairs and pollen tubes by controlling assembly of the cytoskeleton. GhRac1, isolated from cottonseeds, is a member of the Rac/Rop GTPase family and is abundantly expressed in rapidly growing cotton tissues. GhRac1 shows the greatest sequence similarity to the group IV subfamily of Arabidopsis Rac/Rop genes. Overexpression of GhRac1 in E. coli led to the production of a functional GTPase as shown by in vitro enzyme activity assay. In contrast to other Rac/Rop GTPases found in cotton fiber, GhRac1 is highly expressed during the elongation stage of fiber development with expression decreasing dramatically when the rate of fiber elongation declines. The association of highest GhRac1 expression during stages of maximal cotton fiber elongation suggests that GhRac1 GTPase may be a potential regulator of fiber elongation by controlling cytoskeletal assembly.

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Citations

Jan 21, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Doug J HinchliffeBarbara A Triplett
Jun 8, 2012·Genome Génome / Conseil National De Recherches Canada·Ximei LiXianlong Zhang
Sep 17, 2008·BMC Plant Biology·Ibrokhim Y AbdurakhmonovAbdusattor Abdukarimov
Oct 15, 2010·Current Opinion in Plant Biology·Yong-Mei Qin, Yu-Xian Zhu
Oct 15, 2013·Plant Biotechnology Journal·Deepti NigamSamir V Sawant
Apr 11, 2006·Biochimica Et Biophysica Acta·Attila SzucsAttila Fehér
Apr 19, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Ziqiang ChengAndrew Ayrton
Jun 21, 2007·Journal of Experimental Botany·Philippe AbbalCatherine Tesniere
Mar 7, 2019·Frontiers in Plant Science·Guanghui XiaoYu Zhang
Jul 18, 2020·International Journal of Biological Macromolecules·Lichen WangMuhammad Mujtaba

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