PMID: 9185171Jul 1, 1997Paper

Characterization of glutamate and [3H]D-aspartate outflow from various in vitro preparations of the rat hippocampus

Neurochemistry International
A MuzzoliniMichele Simonato

Abstract

The characteristics of high-K+ and electrically evoked endogenous glutamate and [3H]D-aspartate release have been studied in multiple in vitro preparations of the rat hippocampus (transverse slices, granule cells cultures, synaptosomes and mossy fibre synaptosomes) under similar experimental conditions. High external K+ concentrations evoked [3H]D-aspartate and endogenous glutamate overflow in a concentration-dependent manner in all preparations (except it was not possible to measure endogenous glutamate outflow from granule cells). This effect was tetrodotoxin-insensitive but partially calcium-dependent. In slices, field electrical stimulation evoked an overflow of endogenous glutamate, but not of [3H]D-aspartate, in a frequency-dependent manner. This effect was concentration-dependently amplified by the glutamate uptake inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid (t-PDC). The electrically evoked glutamate overflow in the presence of t-PDC was tetrodotoxin-sensitive and calcium-dependent. In primary dentate gyrus cell cultures, electrical stimulation evoked an overflow of [3H]D-aspartate in a frequency-dependent manner, while endogenous glutamate outflow was not detectable. This effect could be inhibited by tetrodotoxi...Continue Reading

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Citations

Aug 9, 2012·Amino Acids·Nobutoshi OtaJonathan V Sweedler
Nov 5, 1999·Trends in Pharmacological Sciences·H P van Helden, T J Bueters
Dec 2, 2000·Neurochemistry International·D D SavageA M Allan
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Mar 5, 2013·Neurobiology of Disease·Silvia ZucchiniMichele Simonato
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Jan 1, 2009·Reviews in the Neurosciences·Francesco ErricoAlessandro Usiello
Nov 16, 2005·Analytical Chemistry·Hai MiaoJonathan V Sweedler

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