PMID: 6125498Sep 1, 1982

Characterization of glutamine-requiring mutants of Pseudomonas aeruginosa

Journal of Bacteriology
D B JanssenC van der Drift

Abstract

Revertants were isolated from a glutamine-requiring mutant of Pseudomonas aeruginosa PAO. One strain showed thermosensitive glutamine requirement and formed thermolabile glutamine synthase, suggesting the presence of a mutation in the structural gene for glutamine synthetase. The mutation conferring glutamine auxotrophy was subsequently mapped and found to be located at about 15 min on the chromosomal map, close to and before hisII4. Furthermore, in transduction experiments, it appeared to be very closely linked to gln-2022, a suppressor mutation affecting nitrogen control. With immunological techniques, it could be demonstrated that the glutamine auxotrophs form an inactive glutamine synthetase protein which is regulated by glutamine or a product derived from it in a way similar to other nitrogen-controlled proteins.

Citations

Dec 17, 2004·Journal of Bacteriology·Vicente García-GonzálezEduardo Santero
Jun 14, 2008·The Journal of Biological Chemistry·Ileana RamazzinaRiccardo Percudani

Related Concepts

Genome Mapping
Chromosomes, Bacterial
Cistron
Genes, Bacterial
D-Glutamine
Glutamate-Ammonia Ligase
Genetic Linkage Analysis
Pseudomonas aeruginosa
Suppressor Mutation
Transduction, Genetic

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