PMID: 7336143Apr 1, 1981Paper

Characterization of Glycophorin A and band 3 from Tn polyagglutinable erythrocytes

Scandinavian Journal of Haematology
M Jokinen

Abstract

The 2 types of erythrocytes from a person with persistent mixed-field polyagglutinability (Tn abnormality) were separated from each other by preparative cell electrophoresis. Surface labelling using the galactose oxidase/NaB3H4 technique followed by polyacrylamide gel electrophoresis showed a strong labelling in the glycophorin A region of Tn positive erythrocytes indicating exposed galactosyl N-acetyl/galactosaminyl residues. Tn positive cell membranes were labelled by the galactose oxidase/NaB3H4 technique and solubilized in non-ionic detergent. After chromatography on Helix pomatia lectin-linked Sepharose, glycophorin A was immunoprecipitated from the sugar eluate using specific antiserum. Glycophorin A from Tn negative cells and normal red blood cells did not bind to Helix pomatia lectin but to Lens culinaris lectin-Sepharose. Glycophorin A and band 3 were isolated by preparative gel electrophoresis from normal cells and the two red cell populations of the Tn individual. Pronase treatment of labelled glycophorin A followed by gel filtration revealed a more efficient proteolysis in molecules isolated from Tn positive cells. Mild alkaline treatment of galactose oxidase/NaB3H4 or periodate/NaB3H4 labelled glycophorin A liberat...Continue Reading

References

Dec 1, 1978·European Journal of Biochemistry·J P CartronA Gerbal
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M Tomita, V T Marchesi
Nov 27, 1974·Biochemical and Biophysical Research Communications·G F Springer, P R Desai
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Oct 1, 1971·British Journal of Haematology·G W BirdJ Wingham

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Citations

Nov 26, 1999·Biochimica Et Biophysica Acta·E G Berger

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