PMID: 9188605Jul 1, 1997Paper

Characterization of glycosylated Gag expressed by a neurovirulent murine leukemia virus: identification of differences in processing in vitro and in vivo

Journal of Virology
R FujisawaJ L Portis

Abstract

The neuroinvasiveness of a chimeric murine retrovirus, CasFrKP (KP), is dependent on the expression of glycosylated Gag (gp85gag). This viral protein is the product of alternate translation initiation 88 codons upstream of and in frame with the initiation codon of pr65gag, the precursor of the viral core proteins. Although expression of glycosylated Gag affects virus spread in the spleen, it appears not to affect virus spread in vitro in fibroblast cell lines (J. L. Portis et al., J. Virol. 68:3879-3887, 1994). The differential effects of this protein in vitro and in vivo have not been explained, and its function is unknown. We have here compared the in vitro processing of this molecule with that expressed in spleens of infected mice. In vitro, gp85gag was cleaved near the middle of the molecule, releasing the C-terminal half (containing capsid and nucleocapsid domains of pr65gag) as a secreted glycoprotein. The N-terminal half of the protein was associated with the plasma membrane as a approximately 55-kDa glycoprotein bearing the matrix domain of pr65gag as well as the N-terminal 88 residue L domain. This processing scheme was also observed in vivo, although two differences were seen. There were differences in N-linked glycos...Continue Reading

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Citations

Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takayuki NittaHung Fan
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Massimo Pizzato
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Spyridon StavrouSusan R Ross
Dec 17, 2014·Journal of Virology·María Carla Rosales GerpeMarc-André Langlois
Jul 16, 2020·MBio·Uddhav TimilsinaSpyridon Stavrou
Oct 31, 2020·Viruses·Karen Salas-BricenoSusan R Ross
Dec 4, 2020·Microorganisms·Wendy Kaichun XuJaquelin P Dudley
May 1, 2021·Viruses·Benjamin StupflerJean-Christophe Paillart

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