PMID: 6406542May 1, 1983Paper

Characterization of glycosylated hemoglobins. Relevance to monitoring of diabetic control and analysis of other proteins

The Journal of Clinical Investigation
R L GarlickH F Bunn

Abstract

Boronate affinity chromatography and ion exchange chromatography were used to measure the levels of glycosylated hemoglobins in normal and diabetic hemolysates, as well as the distribution of glucose adducts on alpha-NH2-valine and epsilon-NH2-lysine residues. When analyzed by ion exchange chromatography on BioRex 70 resin, the Hb Alc peak comprised 4.4 +/- 0.6% of 15 normal hemolysates and 9.1 +/- 2.1% of 15 diabetic hemolysates. The "Hb Alc" was rechromatographed on GlycoGel B boronate affinity resin that binds vicinal hydroxyl groups of covalently linked sugars. Only 70 +/- 5% of the hemoglobin adhered to the resin. Analysis by the thiobarbituric acid colorimetric test confirmed that the affinity resin effectively separated glycosylated from nonglycosylated hemoglobin. When corrected for nonglycosylated contaminants, the mean level of Hb Alc in normal hemolysates was 2.9 +/- 0.4%, a value considerably lower than those previously reported. In addition to Hb Alc, 5.2 +/- 0.5% of the remaining hemoglobin (Hb Ao) was glycosylated. In diabetics, glycosylated Ao was increased in parallel with Hb Alc. After reduction with [3H]borohydride and acid hydrolysis, glycosylated amino acids were first purified on Affi-Gel boronate affinity...Continue Reading

References

Dec 1, 1976·FEBS Letters·R Flückiger, K H Winterhalter
May 1, 1977·The Journal of Clinical Endocrinology and Metabolism·K H GabbayP M Gallop
Jun 1, 1976·The Journal of Clinical Investigation·H F BunnP M Gallop
Aug 19, 1976·The New England Journal of Medicine·R J KoenigA Cerami
Oct 1, 1976·The Journal of Clinical Investigation·J F FitzgibbonsR T Jones
Nov 3, 1975·Biochemical and Biophysical Research Communications·H F BunnP M Gallop
Jul 11, 1968·Biochemical and Biophysical Research Communications·R M Bookchin, P M Gallop
Aug 1, 1966·Biochemistry·W R Holmquist, W A Schroeder
Jan 1, 1981·Annual Review of Biochemistry·M Brownlee, A Cerami
May 1, 1980·European Journal of Biochemistry·L M GarrickH F Bunn
Jan 1, 1982·Alcoholism, Clinical and Experimental Research·H D Hoberman, S M Chiodo
Apr 2, 1981·The New England Journal of Medicine·R FlückigerK H Gabbay
Jun 1, 1980·Diabetologia·M J de BoerA F Casparie
Aug 1, 1980·European Journal of Pediatrics·W Schröter
Aug 1, 1980·Diabetes·D E GoldsteinJ Da Costa
Feb 1, 1981·The Journal of Clinical Investigation·V J StevensC M Peterson

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Citations

Apr 1, 1986·Diabetologia·J N LiangL T Chylack
Aug 1, 1996·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J RamalhoM C Mota
Jun 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y Hayashi, M Makino
Mar 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·S OlufemiD A Robb
Jul 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·P JerntorpJ O Jeppsson
Mar 1, 1993·The International Journal of Biochemistry·M A Sheikh, D A Robb
Jul 5, 1991·Journal of Immunological Methods·L A StewardM P Cohen
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·A I AlayashJ Bonaventura
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A I Alayash, M T Wilson
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A K al-AliD M Power
Mar 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·K M Mohan KumarP Ranganathan
Apr 4, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·B J GouldJ S Yudkin
Dec 1, 1986·Clinical Biochemistry·S Wettre, H von Schenck
Oct 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M TörnqvistP Rydberg
Jun 12, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria Beatriz de la Calle GuntiñasAdela Rosa Rodríguez
Dec 19, 2003·Advances in Renal Replacement Therapy·Thomas Henle, Toshio Miyata
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R ZatzB M Brenner
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J F Poduslo, G L Curran
Aug 21, 2010·The Journal of Biological Chemistry·Sumie MannoYuichi Takakuwa
Jul 28, 2001·Diabetes Technology & Therapeutics·M P Cohen, R S Clements
Nov 1, 1984·The Journal of Clinical Investigation·R L GarlickH F Bunn
Sep 1, 1987·The Journal of Clinical Investigation·F V OrtolaB M Brenner
Jul 24, 1998·Clinical Chemistry and Laboratory Medicine : CCLM·A FinkeJ O Jeppsson
Mar 28, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Jan-Olof JeppssonUNKNOWN International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Jun 12, 2009·Cellular and Molecular Life Sciences : CMLS·Lan ChenRongqiao He
Jan 1, 1991·Free Radical Research Communications·J V Hunt, S P Wolff
Sep 28, 2012·Diabetes & Metabolic Syndrome·Dalia Rafat, Jamal Ahmad
Dec 29, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·V SathiyapriyaSwapnil Gupta
May 21, 2010·Diabetes Care·Diana SherifaliHertzel C Gerstein
Dec 25, 1984·Journal of Molecular Biology·W F Moo-PennA Arnone
Aug 1, 1985·Biochemical Medicine·A KumarT N Pattabiraman
Feb 19, 2015·Journal of Diabetes Science and Technology·Eric P Smith, Robert M Cohen

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