Characterization of granulocyte chemotactic activity from human cytokine-stimulated chondrocytes as interleukin 8

Cytokine
J Van DammeG Opdenakker

Abstract

Human articular chondrocytes, when stimulated with interleukin 1 beta (IL 1 beta), tumor necrosis factor-alpha (TNF-alpha), or with the double stranded RNA poly (rI).poly (rC), produce a chemotactic activity for granulocytes. The induction with IL 1 beta could be abolished by an antibody to IL 1 beta but not by an antibody to interleukin 6 (IL 6), indicating that the latter is not a mediator for the production of chemotactic activity. The inducers had no direct chemotactic effect on granulocytes. The granulocyte chemotactic factor from chondrocytes was characterized with a specific antibody against leukocyte-derived interleukin 8 (IL 8). The specificity of this antibody was demonstrated by immunochemical and biological criteria such that it could immunoprecipitate only the 6-7 kDa IL 8 protein from fibroblasts, and that it did not neutralize a structurally related monocyte chemotactic protein. This antibody against IL 8 completely neutralized the granulocyte chemotactic activity from stimulated chondrocytes. This demonstrates the identity of chondrocyte IL 8 with leukocyte- and fibroblast-derived IL 8. Our data show that leukocyte chemotaxis into the inflamed joint can be mediated by IL 8, induced in both synovial fibroblasts a...Continue Reading

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Citations

Mar 1, 1991·Arthritis and Rheumatism·P R Elford, P H Cooper
Nov 1, 1992·Immunology Today·G Opdenakker, J Van Damme
May 13, 1999·Progress in Histochemistry and Cytochemistry·K Ostergaard, D M Salter
Mar 1, 1992·Equine Veterinary Journal·J S PriceR G Russell
Nov 20, 2002·Infection and Immunity·Abdurrahim KocyigitMustafa Ulukanligil
Oct 31, 1991·Biochemical and Biophysical Research Communications·A FrazerR G Russell
Jul 27, 1992·FEBS Letters·M Baggiolini, I Clark-Lewis
Jan 23, 2016·Cytokine & Growth Factor Reviews·Mieke De BuckJo Van Damme
Jun 1, 1992·Seminars in Arthritis and Rheumatism·G RussellH Skjodt
Jan 1, 1992·Scandinavian Journal of Rheumatology. Supplement·R G RussellA Mackenzie
Mar 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Philippe E Van den SteenGhislain Opdenakker

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