Characterization of grass carp reovirus minor core protein VP4.

Virology Journal
Li-Ming YanQin Fang

Abstract

Grass Carp Reovirus (GCRV), a tentative member in the genus Aquareovirus of family Reoviridae, contains eleven segmented (double-stranded RNA) dsRNA genome which encodes 12 proteins. A low-copy core component protein VP4, encoded by the viral genome segment 5(S5), has been suggested to play a key role in viral genome transcription and replication. To understand the role of minor core protein VP4 played in molecular pathogenesis during GCRV infection, the recombinant GCRV VP4 gene was constructed and expressed in both prokaryotic and mammalian cells in this investigation. The recombinant His-tag fusion VP4 products expressed in E.coli were identified by Western blotting utilizing His-tag specific monoclonal and GCRV polyclonal antibodies. In addition, the expression of VP4 in GCRV infected cells, appeared in granules structure concentrated mainly in the cytoplasm, can be detected by Immunofluorescence (IF) using prepared anti-VP4 polyclonal antibody. Meanwhile, VP4 protein in GCRV core and infected cell lysate was identified by Immunoblotting (IB) assay. Of particular note, the VP4 protein was exhibited a diffuse distribution in the cytoplasm and nucleus in transfected cells, suggesting that VP4 protein may play a partial role i...Continue Reading

References

Aug 5, 2000·Biochemical and Biophysical Research Communications·Q FangJ F Biagini
Nov 14, 2003·The Journal of Biological Chemistry·Jonghwa KimMax L Nibert
Mar 11, 2004·Virus Research·Peter Mertens
Feb 18, 2006·Science in China. Series C, Life Sciences·Qin FangZ H Zhou
Jul 16, 2008·Journal of Molecular Biology·Lingpeng ChengZ Hong Zhou
Jan 30, 2009·Journal of Virology·Takeshi KobayashiTerence S Dermody
Dec 29, 2009·Journal of Molecular Biology·Lingpeng ChengZ Hong Zhou
Jul 6, 2010·Journal of Virological Methods·Lanlan ZhangYaping Wang
Jul 20, 2010·Archives of Virology·Chao FanQin Fang

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Citations

Oct 15, 2013·Archives of Virology·Jing HongliLin Xiangmei
Jan 21, 2021·Frontiers in Cellular and Infection Microbiology·Chao YanKui-Yang Zheng

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Methods Mentioned

BETA
transfection
PCR
ELISA
electrophoresis
density gradient centrifugation
transfect
two-hybrid

Software Mentioned

GCRV image
Pro
Photoshop
Eexpress

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