Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of Botrytis fabae , the Causal Organism of Chocolate Spot of Faba Bean (Vicia faba L.)

Frontiers in Microbiology
Robert C LeeRobert A Syme

Abstract

Chocolate spot is a major fungal disease of faba bean caused by the ascomycete fungus, Botrytis fabae. B. fabae is also implicated in botrytis gray mold disease in lentils, along with B. cinerea. Here we have isolated and characterized two B. fabae isolates from chocolate spot lesions on faba bean leaves. In plant disease assays on faba bean and lentil, B. fabae was more aggressive than B. cinerea and we observed variation in susceptibility among a small set of cultivars for both plant hosts. Using light microscopy, we observed a spreading, generalized necrosis response in faba bean toward B. fabae. In contrast, the plant response to B. cinerea was localized to epidermal cells underlying germinated spores and appressoria. In addition to the species characterization of B. fabae, we produced genome assemblies for both B. fabae isolates using Illumina sequencing. Genome sequencing coverage and assembly size for B. fabae isolates, were 27x and 45x, and 43.2 and 44.5 Mb, respectively. Following genome assembly and annotation, carbohydrate-active enzyme (CAZymes) and effector genes were predicted. There were no major differences in the numbers of each of the major classes of CAZymes. We predicted 29 effector genes for B. fabae, and u...Continue Reading

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Datasets Mentioned

BETA
PRJNA505227

Methods Mentioned

BETA
Illumina sequencing
electrophoresis
RNASeq
light microscopy
RIP

Software Mentioned

NUCmer
TEdenovo
BLASTn
SE
dbCAN2
BLASTp
CD
tBLASTn
Trimmomatic
GeneMark

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