Characterization of gut contractility and microbiota in patients with severe chronic constipation

PloS One
Dina R YarullinaGuzel F Sitdikova

Abstract

Chronic constipation (CC) is one of the most common gastrointestinal disorders worldwide. Its pathogenesis, however, remains largely unclear. The purpose of the present work was to gain an insight into the role of contractility and microbiota in the etiology of CC. To this end, we studied spontaneous and evoked contractile activity of descending colon segments from patients that have undergone surgery for refractory forms of CC. The juxta-mucosal microbiota of these colon samples were characterized with culture-based and 16S rRNA sequencing techniques. In patients with CC the spontaneous colonic motility remained unchanged compared to the control group without dysfunction of intestinal motility. Moreover, contractions induced by potassium chloride and carbachol were increased in both circular and longitudinal colonic muscle strips, thus indicating preservation of contractile apparatus and increased sensitivity to cholinergic nerve stimulation in the constipated intestine. In the test group, the gut microbiota composition was assessed as being typically human, with four dominant bacterial phyla, namely Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria, as well as usual representation of the most prevalent gut bacteri...Continue Reading

References

Oct 1, 1978·The American Journal of Clinical Nutrition·W E MooreL V Holdeman
Jun 9, 1992·Biochemical Pharmacology·A GómezF Sanchez de la Cuesta
Jan 1, 1988·The Journal of Pharmacy and Pharmacology·D E Burleigh
May 20, 1999·The Journal of Physiology·M NeunlistM Schemann
May 11, 2002·Diseases of the Colon and Rectum·Ryouichi TomitaMasahiro Fukuzawa
Jun 1, 2002·Applied and Environmental Microbiology·Hermie J M HarmsenGjalt W Welling
Dec 14, 2002·FEMS Microbiology Letters·Susan E PrydeHarry J Flint
Apr 12, 2003·Diseases of the Colon and Rectum·Gregory P FitzHarrisAnn Lowry
Dec 9, 2003·European Journal of Pharmacology·Kylie J MansfieldElizabeth Burcher
Apr 20, 2004·The American Journal of Gastroenterology·Peter D R Higgins, John F Johanson
Aug 10, 2004·Gastroenterology·Roberto Berni CananiRiccardo Troncone
Sep 30, 2004·The British Journal of Surgery·M E D JarrettM A Kamm
Feb 24, 2005·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Toshimitsu Uchiyama, Russell Chess-Williams
Sep 20, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·I L KhalifI D Maximova
Nov 11, 2005·The American Journal of Gastroenterology·Giovanni BarbaraRoberto Corinaldesi
May 9, 2006·Gastroenterology·John E KellowAndré J P M Smout
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Jun 30, 2007·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Takashi OhamaHiroshi Ozaki
Jul 30, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D WattchowM Costa
Oct 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Harry SokolPhilippe Langella
Aug 4, 2009·Best Practice & Research. Clinical Gastroenterology·Dave Chatoor, Anton Emmnauel
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jul 22, 2010·The American Journal of Gastroenterology·Chin Wen PngTimothy H J Florin
Sep 10, 2010·The ISME Journal·Catherine LozuponeRob Knight
Oct 28, 2010·British Journal of Pharmacology·David A Hall, Christopher J Langmead
Dec 25, 2010·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·Sushil KumarAnoop Saraya
Mar 23, 2012·The ISME Journal·Clara Belzer, Willem M de Vos
May 2, 2012·Obesity·Caroline L J KarlssonKristina Thorngren-Jerneck
Jul 11, 2012·Therapeutic Advances in Gastroenterology·Brian E LacyMichael Crowell
Sep 12, 2012·Nature Reviews. Gastroenterology & Hepatology·Kenton M SandersSean M Ward
Dec 25, 2012·Gastroenterology·Adil E BharuchaAmanda Pressman
Feb 6, 2013·Gastroenterology·Purna C KashyapJustin L Sonnenburg
Feb 7, 2013·Inflammatory Bowel Diseases·Mirjana Rajilić-StojanovićWillem M de Vos
Jul 31, 2014·Physiological Genomics·Lixin ZhuSusan S Baker
Dec 30, 2014·Journal of Neurogastroenterology and Motility·Seong-Eun KimUNKNOWN Constipation Research group of Korean Society of Neurogastroenterology and Motility
Jan 1, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher S ReigstadPurna C Kashyap

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Citations

Oct 29, 2020·Cancer Biotherapy & Radiopharmaceuticals·Meng Gao, Wuliang Yang
Jan 20, 2021·Digestive Diseases and Sciences·Ji-Hong ChenJan D Huizinga
Jan 28, 2021·BMC Gastroenterology·Ilnar F ShaidullovGuzel F Sitdikova
May 1, 2021·Journal of Clinical Medicine·Jakub WłodarczykMarcin Włodarczyk
Aug 14, 2021·Frontiers in Pharmacology·Qi ZhaoYu-Ping Tang

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Datasets Mentioned

BETA
PRJNA590365

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

QIIME
AcqKnowledge
OriginPro

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