Characterization of H+ and HCO3- transporters in CFPAC-1 human pancreatic duct cells

World Journal of Gastroenterology : WJG
Zoltán RakonczayBarry E Argent

Abstract

To characterize H+ and HCO3- transporters in polarized CFPAC-1 human pancreatic duct cells, which were derived from a cystic fibrosis patient with the DeltaF508 CFTR mutation. CFPAC-1 cells were seeded at high density onto permeable supports and grown to confluence. The cells were loaded with the pH-sensitive fluorescent dye BCECF, and mounted into a perfusion chamber, which allowed the simultaneous perfusion of the basolateral and apical membranes. Transmembrane base flux was calculated from the changes in intracellular pH and the buffering capacity of the cells. Our results showed differential permeability to HCO3-/CO2 at the apical and basolateral membranes of CFPAC-1 cells. Na+/ HCO3- co-transporters (NBCs) and Cl-/ HCO3- exchangers (AEs) were present on the basolateral membrane, and Na+/H+ exchangers (NHEs) on both the apical and basolateral membranes of the cells. Basolateral HCO3- uptake was sensitive to variations of extracellular K+ concentration, the membrane permeable carbonic anhydrase (CA) inhibitors acetazolamide (100 micromol/L) and ethoxyzolamide (100 micromol/L), and was partially inhibited by H2-DIDS (600 micromol/L). The membrane-impermeable CA inhibitor 1-N-(4-sulfamoylphenylethyl)-2,4,6-trimethylpyridine pe...Continue Reading

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Citations

Apr 4, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·A K StewartH Ishiguro
Jan 23, 2016·Journal of Dental Research·E BoriG Varga
Jul 27, 2007·Journal of Cellular Physiology·Zoltán RakonczayMichael A Gray
Sep 12, 2009·Pancreas·Irma DemeterBeáta Burghardt
Jul 23, 2015·Tissue Engineering. Part C, Methods·Orsolya HegyesiGábor Varga
Feb 6, 2020·Frontiers in Physiology·Li CaoChristian Stock
Nov 6, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Su Chii KongStine Falsig Pedersen

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