Characterization of H2O2-induced acute apoptosis in cultured neural stem/progenitor cells

FEBS Letters
Hsingchi J LinWu Ma

Abstract

In the present study, we characterized hydrogen peroxide (H2O2)-induced cell apoptosis and related cell signaling pathways in cultured embryonic neural stem/progenitor cells (NS/PCs). Our data indicated that H2O2 induced acute cell apoptosis in NS/PC in concentration- and time-dependent manners and selectively, it transiently increased PI3K-Akt and Mek-Erk1/2 in a dose-dependent manner. Inhibition of PI3K-Akt with wortmannin, a PI3-K inhibitor, was found to significantly increase H2O2-induced acute apoptosis and dramatically decrease basal pGSK3beta levels. The level of pGSK3beta remained unchanged with H2O2 exposure. We conclude that the transient activation of PI3K-Akt signaling delays the H2O2-induced acute apoptosis in cultured NS/PCs in part through maintaining the basal pGSK3beta level and activating other downstream effectors.

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Citations

Aug 13, 2011·Journal of Molecular Neuroscience : MN·Karsten SchwerdtfegerMelitta Schachner
Dec 20, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Shui GuanZhan-Feng Cui
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Feb 23, 2020·Scientific Reports·Kalyn M Rambacher, Nader H Moniri
Oct 1, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Lei Xiang YangYu Zhao
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Jay Pravda
Jul 31, 2020·Cell Stress & Chaperones·Nasrin KhoshlahniMohammad Mohammadzadeh-Vardin
Jul 29, 2006·Bioorganic & Medicinal Chemistry Letters·Nan WangJunYing Miao
Nov 9, 2010·Progress in Neurobiology·Victoria A Rafalski, Anne Brunet

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