Characterization of hapR, a positive regulator of the Vibrio cholerae HA/protease gene hap, and its identification as a functional homologue of the Vibrio harveyi luxR gene

Molecular Microbiology
Michael G Jobling, R K Holmes

Abstract

The Vibrio cholerae HA/protease gene (hap) promoter is inactive in Escherichia coli. We cloned and sequenced the 0.7kb hap promoter fragment from strain 3083-2 and showed that hap is located immediately 3' of ompW, encoding a minor outer membrane protein. A clone from a genomic library of strain 3083-2 was isolated, which was required for activation of the hap promoter in E. coli. Expression from the hap promoter only occurred late in the growth phase. A single complete open reading frame (ORF) designated HapR was identified on a 1.7 kb DNA fragment that was required for activation. Allelic replacements showed that hapR was also essential for hap expression in V. cholerae. In El Tor, but not in classical biotypes of V. cholerae, hapR mutations also produced a rugose colonial phenotype. HapR was shown to encode a 203-amino-acid polypeptide with 71% identity to LuxR of V. harveyi, an essential positive regulator of the lux operon that has no previously identified homologues. The amino-terminal domain (residues 21-68) showed significant homology to the TetR family of helix-turn-helix DNA-binding domains and was 95% identical to the same domain of LuxR. HapR and LuxR activated both the hap and the lux promoters at near wild-type le...Continue Reading

Citations

Jul 9, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·E A Meighen
Jun 19, 2008·Applied Microbiology and Biotechnology·Lei ChenWeihong Jiang
Dec 21, 2000·Biochimica Et Biophysica Acta·Y H LinE A Meighen
Oct 22, 2003·FEMS Microbiology Letters·Mohammed H RashidDavid K R Karaolis
Oct 20, 2009·Nature Chemical Biology·Robert C KellyBonnie L Bassler
Nov 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jun ZhengJohn J Mekalanos
Jun 27, 2012·Journal of Biomolecular Structure & Dynamics·Mobashar Hussain Urf Turabe FazilDurg Vijai Singh
Jun 26, 2012·Nucleic Acids Research·Helen A VincentAnastasia J Callaghan
Oct 20, 2009·Applied and Environmental Microbiology·Hiroaki Hasegawa, Claudia C Häse
Dec 11, 2007·Applied and Environmental Microbiology·Christopher J GrimRita R Colwell
Oct 9, 2003·Applied and Environmental Microbiology·A HülsmannJ D Oliver
Sep 19, 2007·Infection and Immunity·Jyl S MatsonVictor J DiRita
Sep 13, 2001·Infection and Immunity·J A BenitezR A Finkelstein
May 12, 2001·Infection and Immunity·M Sandkvist
Dec 23, 2003·Infection and Immunity·Xin ZhouJames B Kaper
May 24, 2005·Infection and Immunity·James B Kaper, Vanessa Sperandio
Jun 24, 2005·Infection and Immunity·Lihui YuanAnn Progulske-Fox
Jun 16, 2009·Journal of Bacteriology·Nicholas J ShikumaFitnat H Yildiz
Jun 28, 2011·Journal of Bacteriology·Cindy J Gode-Potratz, Linda L McCarter
Jun 12, 2013·Journal of Bacteriology·Yunduan WangJun Zhu
May 29, 2007·Journal of Bacteriology·Rukman S De SilvaF Jon Kull

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