Characterization of heat-stable whey protein: Impact of ultrasound on rheological, thermal, structural and morphological properties

Ultrasonics Sonochemistry
Anju Boora KhatkarNitin Mehta

Abstract

Ultrasound, an emerging non-thermal technique, has potential to modify the functionality of bio-molecules like protein. In the present study, the impact of ultrasound on whey protein (WP) was assessed with functional, rheological, heat coagulation and transition temperature, SDS-PAGE, FTIR spectra, scanning electron microscopy and transmission electron microscopy. The results of this study showed that the raw WP had broad bimodal particle size distribution while after ultrasonication, modified WP exhibited narrower distribution along with smaller particle size (0.683 ± 0.225 μm) compared to untreated WP (2.453 ± 0.717 μm). The solubility of WP also increased after ultrasonication (72.22 ± 0.68% to 79.21 ± 1.08%). During the rheological evaluation, both the samples exhibited Newtonian behaviour but, the modified WP exhibited dramatically higher storage modulus (G') throughout the temperature profile compared to raw WP mainly due to enhanced proteins aggregation during heating which revealed more elastic and stronger gel. The modified WP exhibited significantly higher (about 6-times) heat stability compared to raw WP which signified that after ultrasonication the WP can withstand higher temperature during processing for longer ti...Continue Reading

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Citations

May 26, 2020·Critical Reviews in Food Science and Nutrition·Dacheng KangXing Chen
Jul 3, 2020·Journal of the Science of Food and Agriculture·Feyza Tatar Turan, Talip Kahyaoglu
Aug 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xinhui ZhouMingruo Guo
May 16, 2021·Ultrasonics Sonochemistry·Xuyue WangShugang Li
Oct 6, 2018·Ultrasonics Sonochemistry·Amanda Maria Teixeira LagoJaime Vilela de Resende
Dec 19, 2018·Ultrasonics Sonochemistry·Karen Cristina Guedes Silva, Ana Carla Kawazoe Sato
Nov 9, 2021·Journal of Texture Studies·Raouf AslamRavi Pandiselvam

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