PMID: 45780Jan 1, 1978

Characterization of HeLa 5'-nucleotidase: a stable plasma membrane marker

Membrane Biochemistry
E T BrakeJ S Cook

Abstract

5'-Nucleotidase, assayed as 5'-AMPase, has been extensively characterized and established as a stable, quantitative plasma membrane marker in HeLa S3 cells. The membrane 5'-AMPase has a Km of 7.0 microM. Relative affinities of the other 5'-mononucleotides for the enzyme are 5'-GMP > 5'-TMP > 5'-UMP > 5'-CMP. There are activity optima at pH7 and 10; the latter is Mg(2+)-dependent. The membrane preparations have a small amount of acid phosphatase activity that is distinct from 5'-AMPase activity but no alkaline phosphatase. AOPCP, ADP, and ATP are strongly inhibitory. Mg2+, Ca2+, or Co2+ additions do not affect the pH 7.0 activity; Mn2+ activates slightly, whereas Zn2+, Cu2+, and Ni2+ are inhibitory. EDTA slowly inactivates, but removal of the EDTA without the addition of divalent cations restores activity. The inactivation is also substantially reversed by Co2+ or Mn2+, but reactivability by divalent cations decreases with time in EDTA. ConA strongly inhibits, and alpha-methyl-D-mannoside or glucose (the latter much less efficiently) relieves the inhibition, indicating that the 5'-AMPase is a glycoprotein. Histidine is also inhibitory. Ouabain, phloretin, cytochalasin B, cysteine, phenyl-alanine, MalNEt, and IAA are without effe...Continue Reading

References

Jan 2, 2007·Experimental Cell Research·Abdallah AzouzJonathan S Duke-Cohan
May 1, 1983·Journal of Neurochemistry·J Mallol, J Bozal
Jul 1, 1985·Journal of Neurochemistry·S M RossP S Spencer
Oct 1, 1982·Journal of Neurochemistry·J M Montero, J B Fes
Jan 1, 1982·Journal of Cellular Physiology·J S CookC Shaffer
Jan 1, 1981·Journal of Cellular Physiology·L R PollackJ S Cook
Aug 4, 1998·The American Journal of Physiology·S GaurH M Goodman

Citations

Jan 1, 1976·Archives of Biochemistry and Biophysics·C C Wang, O Touster
May 1, 1976·Canadian Journal of Biochemistry·I H Fox, P J Marchant
Apr 1, 1975·Analytical Biochemistry·M K Gentry, R A Olsson
Nov 1, 1975·Archives of Biochemistry and Biophysics·B L Riemer, C C Widnell
Aug 1, 1975·Journal of Cellular Physiology·J S CookE T Brake
Jul 1, 1972·The Journal of Physiology·P F Baker, J S Willis
Feb 1, 1973·The Journal of Cell Biology·J W DePierre, M L Karnovsky
May 1, 1969·Analytical Biochemistry·R H HintonG C Hartman
Jan 1, 1973·Methods in Cell Biology·P H Atkinson
Apr 29, 1974·Biochimica Et Biophysica Acta·E G Trams, C J Lauter
May 1, 1973·The Biochemical Journal·W H Evans, J W Gurd
Dec 1, 1969·Biochemical Genetics·N A Elson, R P Cox
May 26, 1972·Biochemical and Biophysical Research Communications·M J Hayman, M J Crumpton
May 1, 1972·Laboratory Animals·A I Walker, J W Barrett
Sep 1, 1972·The Journal of Physiology·L J BoardmanD McCall
Jan 1, 1971·Enzyme·P G Hill, H G Sammons
May 5, 1966·Biochimica Et Biophysica Acta·S G AgusM J Griffin
Jan 1, 1969·The Biochemical Journal·A W Murray, B Friedrichs
Oct 1, 1966·The Biochemical Journal·C S Song, O Bodansky
Aug 1, 1953·The Biochemical Journal·M DIXON

Related Concepts

Thermodynamics
Cations, Divalent
Phosphoric Monoester Hydrolases
Histidine
Phenyl
Glycoproteins
NT5E
Alkaline Phosphatase Measurement
MUC7 gene
Ouabain

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.