PMID: 8597381Dec 29, 1995Paper

Characterization of hematopoietic stem cells

Annals of the New York Academy of Sciences
G FritschH Gadner

Abstract

On the basis of density-separated mononuclear cells isolated from bone marrow, peripheral blood, and cord blood, we have repeatedly shown good correlation between two-color flow cytometric (FACS) CD34 analysis and colony formation in the clonogenic assay. We have analyzed the distributions of CD34 subpopulations in these three stem cell sources using patients' and donors' bone marrow biopsies (n = 196), cord blood samples from full-term deliveries (n = 14), and peripheral blood from patients mobilized by chemotherapy and/or cytokine treatment (n = 258). Irrespective of absolute cell counts, the mean (+/- SD) proportions of CD34+ cells were clearly higher in bone marrow (5.6 +/- 4.6% of mononuclear cells) than in peripheral blood (1.9 +/- 2.6) and cord blood (1.7 +/- 2.6). However, two-color FACS analyses revealed significant differences among these cell sources with regard to their distribution of CD34 subpopulations: B-cell progenitors coexpressing CD34 and CD19, at considerable concentrations of > 0.5%, were only found in bone marrow (mean 30 +/- 24.3% of CD34+ mononuclear cells, median 28.7%, minimum 0%, maximum 83.3%). In addition, CD34+ cells in S/G2M phase were never detected in peripheral blood or cord blood, but only in...Continue Reading

Citations

Jun 13, 2008·Nature Protocols·Judy R van BeijnumArjan W Griffioen
Dec 10, 1999·Developmental Biology·J F Amatruda, L I Zon
Jan 1, 1996·International Journal of Health Services : Planning, Administration, Evaluation·R Nixon
Dec 31, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Gina C Schatteman, Ola Awad
Jul 8, 2011·Archives of Pathology & Laboratory Medicine·Alain NgomaKenneth E Nollet

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