Characterization of hemicellulase and cellulase from the extremely thermophilic bacterium Caldicellulosiruptor owensensis and their potential application for bioconversion of lignocellulosic biomass without pretreatment

Biotechnology for Biofuels
Xiaowei PengYejun Han

Abstract

Pretreatment is currently the common approach for improving the efficiency of enzymatic hydrolysis on lignocellulose. However, the pretreatment process is expensive and will produce inhibitors such as furan derivatives and phenol derivatives. If the lignocellulosic biomass can efficiently be saccharified by enzymolysis without pretreatment, the bioconversion process would be simplified. The genus Caldicellulosiruptor, an obligatory anaerobic and extreme thermophile can produce a diverse set of glycoside hydrolases (GHs) for deconstruction of lignocellulosic biomass. It gives potential opportunities for improving the efficiency of converting native lignocellulosic biomass to fermentable sugars. Both of the extracellular (extra-) and intracellular (intra-) enzymes of C. owensensis cultivated on corncob xylan or xylose had cellulase (including endoglucanase, cellobiohydrolase and β-glucosidase) and hemicellulase (including xylanase, xylosidase, arabinofuranosidase and acetyl xylan esterase) activities. The enzymes of C. owensensis had high ability for degrading hemicellulose of native corn stover and corncob with the conversion rates of xylan 16.7 % and araban 60.0 %. Moreover, they had remarkable synergetic function with the comm...Continue Reading

References

May 2, 1998·International Journal of Systematic Bacteriology·C Y HuangL Baresi
Apr 21, 2001·Biotechnology Progress·A MartinezL O Ingram
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Aug 9, 2003·Applied Microbiology and Biotechnology·M J LópezR J Bothast
Jun 16, 2004·Nature Reviews. Microbiology·Roy H Doi, Akihiko Kosugi
Oct 19, 2004·Annual Review of Microbiology·Edward A BayerRaphael Lamed
Dec 14, 2004·Bioresource Technology·Nathan MosierMichael Ladisch
Apr 1, 2006·Bioresource Technology·Chen Hongzhang, Liu Liying
Oct 3, 2006·Bioresource Technology·Anuj Kumar ChandelRamesh Chander Kuhad
Jan 16, 2007·Bioresource Technology·Per SassnerGuido Zacchi
Apr 28, 2007·Current Opinion in Biotechnology·Thaddeus Chukwuemeka EzejiHans Peter Blaschek
Jun 28, 2007·Advances in Biochemical Engineering/biotechnology·Sandra T Merino, Joel Cherry
Feb 9, 2008·Nature Biotechnology·Lee R LyndCharles E Wyman
Jun 6, 2008·Current Opinion in Biotechnology·Sara E Blumer-SchuetteRobert M Kelly
Mar 27, 2009·Applied Biochemistry and Biotechnology·Y-H Percival ZhangBruce E Dale
Apr 3, 2010·Advances in Applied Microbiology·Carl J YeomanIsaac K O Cann
Jun 16, 2010·Biotechnology Advances·Hongzhang Chen, Weihua Qiu
May 27, 2011·Trends in Biotechnology·Alexander V Gusakov
Jun 1, 2011·Biotechnology Advances·Valery B AgborDavid B Levin
Jun 21, 2011·Enzyme Research·Gary BrodeurSubramanian Ramakrishnan
Jan 17, 2012·Applied and Environmental Microbiology·Xiaoyun SuIsaac K O Cann
Nov 27, 2014·Biotechnology for Biofuels·Hong-Zhang Chen, Zhi-Hua Liu
Dec 10, 2014·Biotechnology for Biofuels·Roman BruneckyStephen R Decker

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Citations

Jul 7, 2016·Biotechnology for Biofuels·Chao YangTong Zhang
Oct 6, 2016·Enzyme and Microbial Technology·Fiyinfoluwa A AdesioyeDon A Cowan
Apr 11, 2019·Biotechnology Journal·Jerome AmoahAkihiko Kondo
Jun 7, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nursyafiqah A Mohamad ZabidiRaha Abdul Rahim
Jul 31, 2019·Extremophiles : Life Under Extreme Conditions·Laura L LeeRobert M Kelly
Dec 15, 2020·International Journal of Biological Macromolecules·Nisha SinghMunish Puri
Feb 11, 2021·Plant Biotechnology Journal·Yanrong LiuWanjun Zhang
Aug 1, 2021·Bioresource Technology·Debasree SinhaShibani Chaudhury

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Methods Mentioned

BETA
scanning electron microscopy

Software Mentioned

Bioworks

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