Characterization of HEp-2 cell projection formation induced by diffusely adherent Escherichia coli

Microbial Pathogenesis
S T Cookson, J P Nataro

Abstract

Diffusely adherent Escherichia coli (DAEC) are diarrheagenic E. coli whose pathogenetic mechanisms are largely unknown. DAEC have been shown to induce an unusual phenotype upon adherence to HEp-2 cells in culture characterized by the induction of long thin membrane processes extending from the cell surface. In addition, DAEC have been shown to be protected from the bactericidal effects of gentamicin when incubated with HEp-2 cells. In our studies, we found that three DAEC strains induced formation of eukaryotic cell processes and were protected from gentamicin killing after a 3 h incubation. Preincubation of HEp-2 cells with colchicine or cytochalasin D prior to infection with DAEC strain C1845 resulted in decreased projection formation, suggesting that the effect was dependent upon microfilament and microtubule rearrangement. When the standard gentamicin protection assay was extended for an additional 3 h incubation in the presence of gentamicin, a greater number of DAEC survived gentamicin treatment, more eukaryotic projections were seen in association with the bacteria and the bacteria were actually observed to be "embedded' within these projections. Projection formation was not observed when the bacteria were separated from...Continue Reading

Citations

Apr 16, 2005·Clinical Microbiology Reviews·Alain L Servin
Dec 25, 2004·Infection and Immunity·Alfredo G TorresJames B Kaper
Jun 10, 2006·Molecular Microbiology·Jalaluddin SheikhJames P Nataro
Feb 28, 2006·FEMS Microbiology Letters·Chantal Le Bouguénec, Alain L Servin
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Aug 26, 1998·Infection and Immunity·M G RittigC Bogdan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.