Characterization of hepatocyte-based in vitro systems for reliable toxicity testing

Archives of Toxicology
Mathieu Vinken, Jan G Hengstler

Abstract

A plethora of human hepatocyte-based in vitro systems for toxicity testing has been developed over the past few years. These systems are either directly derived from liver tissue or have been generated through stem cell technology. A wide variety of parameters is currently used to demonstrate the acquisition of in vivo-like hepatocellular physiology and toxicity in such novel in vitro systems. This frequently leads to flawed claims regarding applicability and may impede comparison between in vitro systems. A possible solution lies in defining a set of consensus criteria for proper benchmarking. A proposal for characterization of hepatocyte-based in vitro systems for toxicity screening is made in this paper and consists of testing critical features of viability, morphology, functionality and toxicity.

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Citations

Feb 19, 2020·Archives of Toxicology·Jan G HengstlerJorrit J Hornberg
May 14, 2020·Micromachines·Alican ÖzkanMarissa Nichole Rylander
Jan 27, 2021·Stem Cell Research & Therapy·Meixian JinShuqin Zhou
Feb 7, 2021·International Journal of Environmental Research and Public Health·Neusa FigueiredoMarta Martins
May 30, 2020·Trends in Molecular Medicine·Daniele ZinkJackie Y Ying
Mar 5, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Susana ProençaNynke I Kramer
Mar 19, 2021·Frontiers in Cell and Developmental Biology·Ana S SerrasJoana P Miranda
Apr 19, 2021·Toxicology Letters·Nicholas J ColtmanNikolas J Hodges
Jun 3, 2021·International Journal of Molecular Sciences·Andrés TabernillaMathieu Vinken

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Methods Mentioned

BETA
light microscopy

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