Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis.

Experimental & Applied Acarology
Jinlin ZhouKozo Fujisaki

Abstract

The gene encoding cystatin from the tick Haemaphysalis longicornis has been reported previously. In the study reported here, we characterized a member of cystatins and designated it as Hlcyst-3 (H. longicornis cystatin-3). Its full-length cDNA is 602 bp, and it encodes a putative 129 amino acid protein with an obvious signal peptide. Sequence analysis revealed that it has significant homology with the known secreted cystatin. The recombinant protein was expressed in a GST-fused soluble form in Escherichia coli and was purified by affinity chromatography. The inhibitory activity of the recombinant protein against papain and cathepsin L was identified by fluorogenic substrate analysis. Real-time PCR revealed that Hlcyst-3 was mostly expressed in the tick midgut.

References

Jul 22, 1991·FEBS Letters·V Turk, W Bode
Jan 1, 1990·Journal of Molecular Evolution·N D Rawlings, A J Barrett
Jan 1, 1997·Protein Science : a Publication of the Protein Society·W M Brown, K M Dziegielewska
Jun 24, 1999·The Journal of Veterinary Medical Science·A MulengaM Onuma
Sep 30, 1999·Archives of Insect Biochemistry and Physiology·Y YamamotoS Y Takahashi
Dec 11, 1999·Nucleic Acids Research·J SchultzP Bork
Jan 1, 1994·Parasitology Today·K FujisakiT Kamio
Jun 9, 2005·Parasitology·F Jongejan, G Uilenberg
Jul 26, 2005·Biochemical and Biophysical Research Communications·Shahid KarimThomas N Mather
Jun 15, 2006·The Journal of Biological Chemistry·Michalis KotsyfakisJosé M C Ribeiro
Jun 30, 2006·Biochemical and Biophysical Research Communications·Cassia A LimaAparecida S Tanaka
Aug 19, 2007·The Journal of Biological Chemistry·Michalis KotsyfakisJosé M C Ribeiro
May 17, 2008·PLoS Pathogens·Naotoshi TsujiKozo Fujisaki
Dec 19, 2008·Veterinary Parasitology·Jinlin ZhouKozo Fujisaki

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Citations

Oct 12, 2012·Experimental & Applied Acarology·Adriana Mércia Guaratini IbelliAlbert Mulenga
Sep 17, 2013·Ticks and Tick-borne Diseases·Luís F PariziItabajara da Silva Vaz
Jun 1, 2012·Ticks and Tick-borne Diseases·Alexandra SchwarzMichalis Kotsyfakis
Apr 19, 2015·Parasites & Vectors·Luís Fernando PariziItabajara da Silva Vaz
Nov 8, 2017·Medical and Veterinary Entomology·L F PariziI DA Silva Vaz
Feb 10, 2018·Parasites & Vectors·Lei LiuTian-Yin Cheng
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Jindřich ChmelařMichail Kotsyfakis
Dec 10, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Larissa Almeida MartinsMichail Kotsyfakis

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