PMID: 6171265Apr 15, 1981Paper

Characterization of hormonally regulated secretory proteins from the caput epididymidis of the rabbit

The Biochemical Journal
R JonesC R Brown

Abstract

1. The incorporation of [35S]methionine into protein was investigated in tissue minces from different regions of the rabbit epididymis incubated in vitro. Rates of synthesis were in the order: epididymal regions 2-5 greater than region 7 greater than region 6 greater than region 1 greater than region 8 greater than ductus deferens greater than ductuli efferentes. 2. Separation of labelled proteins on polyacrylamide gels containing sodium dodecyl sulphate followed by fluorography revealed that one protein (mol.wt. approx. 90 000) was characteristic of region 1, four proteins (one of mol.wt. 54 000 and three of mol.wt. 20 000) were synthesized principally in regions 2-5, and one protein (mol.wt. 22 500) was produced mainly in regions 6, 7 and 8. 3. Castration for 14 days decreased incorporation of [35S]methionine into total protein to less than 10% of that in controls in all regions of the epididymis. However, testosterone treatment for a further period of 14 days restored protein synthesis to normal values in regions 6, 7 and 8, but not in region 1 or regions 2-5. In regions 2-5 the synthesis of three proteins of mol.wt. 20 000 declined after castration, but was not stimulated by exogenous testosterone. Since the 20 000-mol.wt. ...Continue Reading

Citations

Jan 18, 2008·Biology of Reproduction·Brett NixonMichael K Holland
Jun 25, 2002·Biology of Reproduction·Brett NixonMichael K Holland
May 1, 1989·The Journal of Experimental Zoology·F Regalado, A Nieto
May 1, 1995·Molecular Reproduction and Development·G A Cornwall, S R Hann
Nov 1, 1985·The American Journal of Anatomy·E M EddyB A Fenderson
Apr 1, 1991·The Journal of Experimental Zoology·G RobitailleG Bleau
Sep 1, 1987·The American Journal of Anatomy·M C Orgebin-CristM D Skudlarek
May 1, 1990·Molecular Reproduction and Development·P RossK D Roberts
Dec 1, 1993·Molecular Reproduction and Development·F RegaladoA Nieto
Jan 1, 1989·Archives of Andrology·D K Kapur, G K Ahuja
Mar 1, 1994·American Journal of Reproductive Immunology : AJRI·M C MahonyG D Hodgen
Jan 1, 1990·Archives of Andrology·G C MajumderM Barua
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A DepeigesJ P Dufaure

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