PMID: 9545528Apr 18, 1998Paper

Characterization of HSE sequences in human Hsp40 gene: structural and promoter analysis

Biochimica Et Biophysica Acta
M Hata, K Ohtsuka

Abstract

We have recently cloned a gene of Hsp40, a human homologue of bacterial DnaJ. Here we describe the structural and promoter analysis of human Hsp40 gene. Analysis of Hsp40 transcripts by 5' and 3' RACE suggested that they have different 3' ends, and primer extension studies revealed that the major transcription initiation site was localized 47 bp upstream of the ATG translation initiation codon. Promoter analysis using deletion derivatives defined a minimal region which was active in response to heat shock. The region contained the consensus heat shock element (HSE) sequences. The factor bound to these sequences was suggested to be a heat shock factor 1 (HSF1) by gel mobility supershift assay. In vivo footprinting and promoter analysis revealed that the HSEs in 5' upstream region of human Hsp40 gene were composed of eight contiguous (A/G)GAAN motifs and were essential for heat shock response. These results indicate that Hsp40 is a real heat shock protein. It is also shown that the HSE found in the first intron might not be the essential element for heat shock response.

References

Aug 1, 1992·Trends in Biochemical Sciences·C Georgopoulos
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K RabindranC Wu
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T J SchuetzR E Kingston
Jan 30, 1990·Biochemical and Biophysical Research Communications·K OhtsukaK Nagata
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·W Gilbert
Jan 1, 1988·Annual Review of Genetics·S Lindquist, E A Craig
Sep 1, 1988·Molecular and Cellular Biology·J AminR Voellmy
Apr 1, 1994·Trends in Biochemical Sciences·D M CyrM G Douglas
Jan 1, 1993·Annual Review of Biochemistry·J P Hendrick, F U Hartl
Nov 30, 1993·Biochemical and Biophysical Research Communications·K Ohtsuka
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·M W MelvilleM G Katze
Mar 1, 1985·Mycotoxin Research·H M StahrR Pfeiffer

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Citations

Apr 1, 2009·Cell Stress & Chaperones·Daisuke KawashimaKenzo Ohtsuka
Sep 20, 2011·Cancer Letters·Jason N SterrenbergAdrienne L Edkins
Sep 16, 2017·International Journal of Molecular Sciences·Suman Chatterjee, Timothy F Burns
Oct 20, 2006·Journal of Biochemistry·Yukako UchiyamaKazutoyo Terada
Nov 25, 2000·DNA Sequence : the Journal of DNA Sequencing and Mapping·M Hata, K Ohtsuka
Apr 18, 2001·The Journal of Biological Chemistry·C ImbrianoR Mantovani
Feb 24, 2000·Biochimica Et Biophysica Acta·L F CooperZ Guo

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