PMID: 2125268Dec 12, 1990Paper

Characterization of human factor VIII and interaction with von Willebrand factor. An electron microscopic study

European Journal of Biochemistry
H F HeijnenJ J Sixma

Abstract

Blood coagulation factor VIII is a large glycoprotein that circulates in plasma at relative low concentration (0.1 microgram/ml). It consists of a heterogeneous mixture of a series heavy-chain peptides (90-200 kDa), each associated with a light chain of 80 kDa. To gain insight into the physical properties of the protein, we have characterized purified human factor VIII by electron microscopy and rotary shadowing. Electron microscopy of rotary shadowed factor VIII molecules showed predominantly a single globular domain structure, with a somewhat asymmetric shape, while two-domain structures were also encountered. The overall dimensions of the globular domains ranged from 4 x 6 nm to 8 x 12 nm. EDTA treatment of factor VIII reduced the overall dimensions (2.5 x 5 nm to 6 x 10 nm) while treatment with thrombin reduced the dimensions to a small extent. In complexes with von Willebrand factor, factor VIII appeared localized at the globular domains of von Willebrand factor multimers. In addition, incubation of factor VIII with Staphylococcus aureus V8 protease fragments SpII and SpIII revealed only binding to the globular domains of SpIII. In this study, the first morphological characterization of human factor VIII is presented, toge...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 24, 1989·Biochemistry·P Lollar, C G Parker
Oct 1, 1985·The Journal of Clinical Investigation·W E FowlerP A McKee
Mar 1, 1988·Trends in Biochemical Sciences·K Titani, K A Walsh
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L O AnderssonJ Smart
Jul 1, 1987·European Journal of Biochemistry·R J HamerJ J Sixma
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R J JennyK G Mann
Sep 1, 1987·European Journal of Biochemistry·R J HamerJ J Sixma
Apr 1, 1985·Journal of Ultrastructure Research·A P MouldJ A Chapman
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K M BrinkhousM S Read
Dec 31, 1985·Biochemistry·P LollarD N Fass
Dec 22, 1970·Biochimica Et Biophysica Acta·J A van Mourik, I A Mochtar
May 1, 1980·Journal of Ultrastructure Research·J M Tyler, D Branton
Nov 22, 1984·Nature·G A VeharD J Capon
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W R ChurchD N Fass
Apr 1, 1982·The Journal of Clinical Investigation·M B Hultin

❮ Previous
Next ❯

Citations

Aug 30, 2002·Haemophilia : the Official Journal of the World Federation of Hemophilia·A LindgrenL Tengborn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.