Characterization of human mitochondrial RNase P: novel aspects in tRNA processing

Biochemical and Biophysical Research Communications
W Rossmanith, R M Karwan

Abstract

Human mitochondrial RNase P does not distinguish itself from other RNase P enzymes by most of its basic properties. 5' phosphates on tRNA products, strict dependence on a divalent cation, independence of ATP or other cofactors, and sensitivity to puromycin are generally characteristic for RNase P. Slow sedimentation of human mitochondrial RNase P in glycerol gradients suggests a molecular weight considerably lower than that of bacterial or nuclear RNase P. In contrast to fungi, all putative components of mammalian mitochondrial RNase P are encoded by the nucleus. Intriguingly, no indication of the involvement of a trans-acting RNA was found in mammalian mitochondrial tRNA processing. Mitochondrial RNase P is resistant to rigorous treatments with nucleases and exhibits a protein-like density in Cs2SO4 gradients. Moreover, an analysis of copurifying RNAs revealed no putative RNase P RNA candidates. These data suggest that mammalian mitochondrial RNase P, unlike its nuclear counterpart or its bacterial relatives, is not a ribonucleoprotein but a protein enzyme.

References

May 1, 1992·Trends in Biochemical Sciences·S C DarrN R Pace
May 11, 1991·Nucleic Acids Research·M DoriaG P Tocchini-Valentini
Jan 1, 1991·Annual Review of Cell Biology·D A Clayton
Jul 1, 1990·Journal of Medical Entomology·J B Morales-Malacara, R López
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·N LawrenceS Altman
Aug 12, 1969·Biochimica Et Biophysica Acta·G H Barlow, E Margoliash
Jan 1, 1980·Methods in Enzymology·T E EnglandO C Uhlenbeck
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris
Jan 1, 1984·Methods in Enzymology·C R MerrilM L Van Keuren
Apr 9, 1981·Nature·S AndersonI G Young
Apr 1, 1995·Journal of Bacteriology·N R Pace, J W Brown
Jul 11, 1993·Nucleic Acids Research·T PotuschakR Karwan
Aug 1, 1993·Journal of Bacteriology·T E LaGrandeurN R Pace
Mar 15, 1995·European Journal of Biochemistry·C StathopoulosD Drainas
May 26, 1995·The Journal of Biological Chemistry·W RossmanithE Sbisà
Aug 1, 1995·Molecular Microbiology·L A Kirsebom
Jan 15, 1996·European Journal of Biochemistry·Y C LeeH S Kang
Jan 15, 1996·European Journal of Biochemistry·Y C LeeH S Kang
Jul 12, 1996·The Journal of Biological Chemistry·H L True, D W Celander

❮ Previous
Next ❯

Citations

Mar 10, 2001·Journal of Cellular Physiology·S XiaoD R Engelke
Jan 6, 2001·RNA·S AltmanA Vioque
Jan 17, 2013·Nature Communications·Anthony GobertPhilippe Giegé
May 3, 2012·Genes & Development·Bernard GutmannPhilippe Giegé
Sep 3, 2010·Genes & Development·Eric M Phizicky, Anita K Hopper
Sep 10, 2008·Molecular and Cellular Biology·Melissa S SchonauerCarol L Dieckmann
Nov 17, 2001·Molecular and Cellular Biology·W Rossmanith, T Potuschak
Jun 5, 2002·Annual Review of Biochemistry·Shaohua XiaoDavid R Engelke
Oct 22, 2009·Journal of Cellular Biochemistry·Michael C Marvin, David R Engelke
Dec 6, 2011·Biochimica Et Biophysica Acta·Walter Rossmanith
Aug 9, 2013·RNA Biology·Franziska PinkerPhilippe Giegé
Dec 8, 2016·The FEBS Journal·Francesco BruniZofia M Chrzanowska-Lightowlers
May 1, 2015·Scientific Reports·Fengzhi LiYuequan Shen
May 5, 2011·Journal of Inherited Metabolic Disease·Adriana P RebeloCarlos T Moraes
Aug 19, 2007·Molecular BioSystems·Sidney Altman
Apr 6, 2006·Critical Reviews in Biochemistry and Molecular Biology·Scott C Walker, David R Engelke
Aug 11, 2006·American Journal of Physiology. Cell Physiology·Shaharyar M KhanRussell H Swerdlow
Sep 12, 2001·The Journal of Biological Chemistry·N S EntelisI A Tarassov
Nov 4, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Franziska PinkerClaude Sauter
Aug 25, 2017·Epigenetics & Chromatin·Yaru DongJizhe Cui
May 17, 2008·Biochemical Society Transactions·Agnieszka J BobrowiczZofia Chrzanowska-Lightowlers
Jun 17, 1999·FEMS Microbiology Reviews·A Schön
Dec 21, 2000·Trends in Cell Biology·A Schneider, L Maréchal-Drouard
May 30, 2001·Molecular and Biochemical Parasitology·R SalavatiK D Stuart
May 30, 2001·Advanced Drug Delivery Reviews·N S EntelisI A Tarassov
Feb 5, 2008·Methods : a Companion to Methods in Enzymology·Marie SisslerCatherine Florentz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.